Gonadotropin-releasing hormone receptor gene expression in human ovary and granulosa-lutein cells
| Autor: | Spyros N. Pavlou, Demetrios Minaretzis, M Jakubowski, Joseph F. Mortola |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
endocrine system medicine.medical_specialty Transcription Genetic medicine.drug_class Endocrinology Diabetes and Metabolism medicine.medical_treatment Molecular Sequence Data Clinical Biochemistry Gene Expression Ovary Gonadotropin-releasing hormone Biology Biochemistry Ribonucleases Endocrinology Anterior pituitary Luteal Cells Internal medicine Follicular phase medicine Humans RNA Messenger Granulosa Lutein Cell Granulosa Cells In vitro fertilisation Base Sequence Biochemistry (medical) GNRHR Nucleic Acid Hybridization Middle Aged medicine.anatomical_structure Molecular Probes Female Gonadotropin Receptors LHRH hormones hormone substitutes and hormone antagonists |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 80:430-434 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.80.2.7852501 |
| Popis: | GnRH regulates gonadotropin biosynthesis and release in the anterior pituitary via specific receptors. Although extrapituitary expression and action of GnRH have been shown in some species, in the human it is not clear whether GnRH has a peripheral action. In this study we sought to determine whether the human ovary expresses GnRH receptor (GnRHR) messenger ribonucleic acid (mRNA). Ovarian tissues from 11 women (32-61 yr old) and granulosa-lutein (GL) cells purified from follicular aspirates of 51 women undergoing oocyte retrieval for in vitro fertilization were analyzed by ribonuclease protection assay and reverse transcriptase-polymerase chain reaction (RT-PCR). Human pituitaries, lymphocytes, and placenta were also studied. Measurable levels of GnRHR mRNA were found by ribonuclease protection assay in 2 of 10 ovaries, in 2 of 4 GL cells preparations from women whose ovarian hyperstimulation involved a GnRH agonist, in GL cells from 3 women whose ovarian hyperstimulation involved a GnRH antagonist, and in human pituitaries. Relative to the total amount of RNA analyzed, the level of GnRHR mRNA was about 200-fold lower in the ovary than in the pituitary. A sequence of 314 basepairs of GnRHR mRNA was amplified by RT-PCR in the pituitary, in 9 of 10 ovaries, and in 4 of 5 GL cell preparations. No message could be amplified in human lymphocytes, and placental specimens showed a weak signal. The relative GnRHR mRNA levels in GL cells from 13 women analyzed by quantitative RT-PCR showed a wide range of individual differences. These results suggest that GnRHR mRNA is expressed in GL cells and the human ovary across different functional stages, implying that multiple ovarian compartments may express GnRH receptors. The administration of GnRH analogs may have a further direct action on the human ovary. |
| Databáze: | OpenAIRE |
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