Osteochondral Regeneration with 3D‐Printed Biodegradable High‐Strength Supramolecular Polymer Reinforced‐Gelatin Hydrogel Scaffolds
| Autor: | Ziyang Xu, Changshun Ruan, Xu Cui, Haofei Li, Fei Gao, Xiaoli Zhao, Wu Mingming, Liuqi Peng, Wenguang Liu, Qingfei Liang |
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| Rok vydání: | 2019 |
| Předmět: |
Scaffold
food.ingredient Materials science General Chemical Engineering osteochondral regeneration General Physics and Astronomy Medicine (miscellaneous) Modulus 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Gelatin food biohybrid gradient scaffolds high strength Ultimate tensile strength General Materials Science lcsh:Science supramolecular polymers chemistry.chemical_classification Full Paper Regeneration (biology) technology industry and agriculture General Engineering 3D printing Full Papers 021001 nanoscience & nanotechnology 0104 chemical sciences Supramolecular polymers Compressive strength chemistry Polymerization lcsh:Q 0210 nano-technology Biomedical engineering |
| Zdroj: | Advanced Science, Vol 6, Iss 15, Pp n/a-n/a (2019) Advanced Science |
| ISSN: | 2198-3844 |
| DOI: | 10.1002/advs.201900867 |
| Popis: | Biomacromolecules with poor mechanical properties cannot satisfy the stringent requirement for load‐bearing as bioscaffolds. Herein, a biodegradable high‐strength supramolecular polymer strengthened hydrogel composed of cleavable poly(N‐acryloyl 2‐glycine) (PACG) and methacrylated gelatin (GelMA) (PACG‐GelMA) is successfully constructed by photo‐initiated polymerization. Introducing hydrogen bond‐strengthened PACG contributes to a significant increase in the mechanical strengths of gelatin hydrogel with a high tensile strength (up to 1.1 MPa), outstanding compressive strength (up to 12.4 MPa), large Young's modulus (up to 320 kPa), and high compression modulus (up to 837 kPa). In turn, the GelMA chemical crosslinking could stabilize the temporary PACG network, showing tunable biodegradability by adjusting ACG/GelMA ratios. Further, a biohybrid gradient scaffold consisting of top layer of PACG‐GelMA hydrogel‐Mn2+ and bottom layer of PACG‐GelMA hydrogel‐bioactive glass is fabricated for repair of osteochondral defects by a 3D printing technique. In vitro biological experiments demonstrate that the biohybrid gradient hydrogel scaffold not only supports cell attachment and spreading but also enhances gene expression of chondrogenic‐related and osteogenic‐related differentiation of human bone marrow stem cells. Around 12 weeks after in vivo implantation, the biohybrid gradient hydrogel scaffold significantly facilitates concurrent regeneration of cartilage and subchondral bone in a rat model. |
| Databáze: | OpenAIRE |
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