Seed defensins of barnyard grass Echinochloa crusgalli (L.) Beauv
| Autor: | Tatyana I. Odintsova, Eugene V. Grishin, Tsezi A. Egorov, Nasser Yalpani, A. K. Musolyamov, Yurij Baranov, Eugene A. Rogozhin |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Antifungal
Antifungal Agents Phytophthora infestans medicine.drug_class Molecular Sequence Data Echinochloa crusgalli Biochemistry Defensins Anti-Infective Agents Botany medicine Amino Acid Sequence Single amino acid Gene Oomycete chemistry.chemical_classification integumentary system biology fungi Fungi hemic and immune systems General Medicine respiratory system bacterial infections and mycoses biology.organism_classification Amino acid Amino Acid Substitution chemistry Echinochloa Seeds Weed Sequence Alignment |
| Zdroj: | Biochimie. 90:1667-1673 |
| ISSN: | 0300-9084 |
| Popis: | From the annual weed barnyard grass Echinochloa crusgalli (L.) Beauv., two novel defensins Ec-AMP-D1 and Ec-AMP-D2 that differ by a single amino acid substitution were isolated by a combination of different chromatographic procedures. Both defensins were active against several phytopathogenic fungi and the oomycete Phytophthora infestans at micromolar concentrations. The Ec-AMP-D1 showed higher activity against the oomycete than Ec-AMP-D2. The comparison of the amino acid sequences of the antifungal E. crusgalli defensins with those of earlier characterized T. kiharae defensins [T.I. Odintsova, Ts.A. Egorov, A.Kh. Musolyamov, M.S. Odintsova, V.A. Pukhalsky, E.V. Grishin, Seed defensins from T. kiharae and related species: genome localization of defensin-encoding genes, Biochimie, 89 (2007) 605–612.] that were devoid of substantial antifungal activity point to the C-terminal region of the molecule as the main determinant of the antifungal activity of E. crusgalli defensins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect