Analgesic, Anti-Inflammatory, and Chondroprotective Activities ofCryptolepis buchananiExtract:In VitroandIn VivoStudies
| Autor: | Vichai Reutrakul, Supanimit Teekachunhatean, Samreang Bunteang, Ampai Panthong, Rujirek Chaiwongsa, Siriwan Ongchai, Chaichan Sangdee, Nutthiya Hanprasertpong, Puongtip Kunanusorn, Narong Nathasaen |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Article Subject Swine medicine.drug_class Anti-Inflammatory Agents Pain lcsh:Medicine Arthritis Osteoarthritis Pharmacology General Biochemistry Genetics and Molecular Biology Anti-inflammatory Rats Sprague-Dawley Mice Chondrocytes In vivo Muscle tension medicine Animals Viability assay Cells Cultured Inflammation Analgesics General Immunology and Microbiology Plant Extracts business.industry Cartilage lcsh:R Cryptolepis General Medicine medicine.disease Rats medicine.anatomical_structure Cytoprotection Immunology business Research Article Explant culture |
| Zdroj: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2014/978582 |
| Popis: | Cryptolepis buchananiRoem. & Schult. is widely used in folk medicine in Southeast Asia for treating muscle tension and arthritis. This study aimed to investigate an analgesic activity of the methanol extract ofC. buchanani(CBE) in acetic acid-induced writhing response in mice, and to examine its anti-inflammatory activity in ethyl phenylpropiolate- (EPP-) induced ear edema and carrageenan-induced paw edema in rats. Its effects on cartilage degradation induced by interleukin-1β(IL-1β) in porcine cartilage explant culture were also determined. This study demonstrated that CBE significantly reduced acetic acid-induced writhing response. It also inhibited edema formation in both EPP-induced ear edema and carrageenan-induced paw edema models. In cartilage explant culture, CBE significantly reduced the sulfated glycosaminoglycan and hyaluronan released into culture media while it reserved the uronic acid and collagen within the cartilage tissues. It also suppressed the matrix metalloproteinase-2 activity with no effect on cell viability. In conclusion, CBE shows analgesic, anti-inflammatory, and chondroprotective effects in this preliminary study. Therefore, CBE may be useful as an alternative treatment for osteoarthritis. |
| Databáze: | OpenAIRE |
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