Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis
| Autor: | P Renault, S D Ehrlich, S R Swindell, Michael J. Gasson, K H Benson, H G Griffin |
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| Přispěvatelé: | Unité de recherche Génétique Microbienne (UGM), Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Carboxy-lyases Carboxy-Lyases Molecular Sequence Data Gene Expression Diacetyl Molecular cloning medicine.disease_cause Applied Microbiology and Biotechnology chemistry.chemical_compound medicine LACTOCOCCUS LACTIS Cloning Molecular DNA Primers [SDV.EE]Life Sciences [q-bio]/Ecology environment Mutation Ecology biology Base Sequence Acetoin Lactococcus lactis food and beverages biology.organism_classification Molecular biology Lactic acid [SDV.EE] Life Sciences [q-bio]/Ecology environment Acetolactate Synthase chemistry Biochemistry Genes Bacterial Lactates CLONAGE DE GENE Homologous recombination Gene Deletion Food Science Biotechnology Research Article |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 1996, 62 (7), pp.2641-2643 |
| ISSN: | 0099-2240 1098-5336 |
| Popis: | Diacetyl is an important food flavor compound produced by certain strains of citrate-metabolizing lactic acid bacteria. Citrate is converted to pyruvate, from which diacetyl is produced via intermediate alpha-acetolactate. This paper reports the cloning and analysis of the gene (aldB) encoding alpha-acetolactate decarboxylase from Lactococcus lactis MG1363. Deletion of the MG1363 chromosomal aldB gene was achieved by double crossover homologous recombination. The mutant strain was found to produce diacetyl; alpha-acetolactate decarboxylase activity was eliminated. Overexpression of the cloned ilvBN genes (encoding an alpha-acetolactate synthase) in the aldB deletion strain produced even higher levels of alpha-acetolactate, acetoin, and diacetyl. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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