The Simple One-Step (SOS) Stool Processing Method for Use with the Xpert MTB/RIF Assay for a Child-Friendly Diagnosis of Tuberculosis Closer to the Point of Care
| Autor: | de Haas, Petra, Yenew, Bazezew, Mengesha, Endale, Slyzkyi, Andrii, Gashu, Zewdu, Lounnas, Manon, Tesfaye, Ephrem, Bedru, Ahmed, Tiemersma, Edine, Kremer, Kristin, Amare, Misikir, Diriba, Getu, Zerihun, Betselot, Gudina, Tilaye, Tegegn, Ben, Bonnet, Maryline, Negeri, Challa, Klinkenberg, Eveline |
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| Přispěvatelé: | Ethiopian Public Health Institute (EPHI), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes (TransVIHMI), Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Amsterdam UMC - Amsterdam University Medical Center |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty MESH: Mycobacterium tuberculosis Tuberculosis Point-of-Care Systems Xpert MTB/RIF MESH: Ethiopia Sensitivity and Specificity fluids and secretions MESH: Sputum Pulmonary tuberculosis Internal medicine Diagnosis Simple one-step stool method Humans Medicine MESH: Tuberculosis Children Point of care MESH: Humans biology business.industry Bacteriologically confirmed MESH: Child Preschool Sputum Mycobacteriology and Aerobic Actinomycetes Mycobacterium tuberculosis medicine.disease biology.organism_classification MESH: Sensitivity and Specificity MESH: Point-of-Care Systems Processing methods [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Mycobacterium tuberculosis complex Stool Child Preschool Ethiopia medicine.symptom business |
| Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, 2021, 59 (8), pp.e0040621. ⟨10.1128/jcm.00406-21⟩ |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | International audience; Young children cannot easily produce sputum for diagnosis of pulmonary tuberculosis (TB). Alternatively, Mycobacterium tuberculosis complex bacilli can be detected in stool by using the Xpert MTB/RIF (Ultra) assay (Xpert). Published stool processing methods contain somewhat complex procedures and require additional supplies. The aim of this study was to develop a simple one-step (SOS) stool processing method based on gravity sedimentation only, similar to Xpert testing of sputum samples, for the detection of M. tuberculosis in stool samples. We first assessed whether the SOS stool method could provide valid Xpert results without the need for bead-beating, dilution, and filtration steps. We concluded that this was the case, and we then validated the SOS stool method by testing spiked stool samples. By using the SOS stool method, 27 of the 29 spiked samples gave valid Xpert results, and M. tuberculosis was recovered from all 27 samples. The proof of principle of the SOS stool method was demonstrated in routine settings in Addis Ababa, Ethiopia. Nine of 123 children with presumptive TB had M. tuberculosis-positive results for nasogastric aspiration (NGA) samples, and 7 (77.8%) of those children also had M. tuberculosis-positive Xpert results for stool samples. Additionally, M. tuberculosis was detected in the stool samples but not the NGA samples from 2 children. The SOS stool processing method makes use of the standard Xpert assay kit, without the need for additional supplies or equipment. The method can potentially be rolled out to any Xpert site, bringing a bacteriologically confirmed diagnosis of TB in children closer to the point of care. |
| Databáze: | OpenAIRE |
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