Effects of Humanin on Experimental Colitis Induced by 2,4,6-trinitrobenzene Sulphonic Acid in Rats
| Autor: | Ummuhani Ozel Turkcu, Fatma Ayca Gultekin, Ufuk Tali, Figen Barut, Demet Sümer, Sevim Karakaş Çelik, Ali Ugur Emre |
|---|---|
| Přispěvatelé: | MÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Özel Türkcü, Ümmühani, Zonguldak Bülent Ecevit Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Interleukin-1beta humanin Anti-Inflammatory Agents Endogeny Inflammation Apoptosis Pharmacology 03 medical and health sciences chemistry.chemical_compound Weight Loss medicine Ulcerative Colitis Animals lcsh:RC799-869 Colitis Intestinal Mucosa Humanin ulcerative colitis Ethanol business.industry Caspase 3 Tumor Necrosis Factor-alpha Gastroenterology Intracellular Signaling Peptides and Proteins medicine.disease Ulcerative colitis Rats 030104 developmental biology Treatment Outcome chemistry Gene Expression Regulation Trinitrobenzenesulfonic Acid Immunology lcsh:Diseases of the digestive system. Gastroenterology Tumor necrosis factor alpha Original Article Colitis Ulcerative medicine.symptom business |
| Zdroj: | Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association The Saudi Journal of Gastroenterology, Vol 23, Iss 2, Pp 105-111 (2017) |
| ISSN: | 1998-4049 1319-3767 |
| Popis: | Background/Aim: The excessive apoptosis of intestinal epithelial cells (IECs) partly accounts for the development of colonic inflammation and eventually results in ulcerative colitis (UC). Humanin, an endogenous anti-apoptotic peptide, has previously been shown to protect against Alzheimer's disease and a variety of cellular insults. The present study aimed to investigate the effects of glysin variant of humanin (HNG) on 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats. Materials and Methods: Rats were divided into four groups as follows: Group 1 (n = 8): control; isotonic saline solution 0.1 ml/rat rectally, Group 2 (n = 8): TNBS colitis; 0.1 ml of a 2.5% (w/v) TNBS solution in 50% ethanol rectally, Group 3 (n = 8): 10 µM HNG, and Group 4 (n = 8): 20 µM HNG intraperitoneal (ip) on day 2 and 6 after rectal TNBS administration. Rats were sacrificed 7 days after the induction of colitis. Blood and tissue samples were harvested for biochemical and histopathological analysis. Results: HNG treatment significantly ameliorated weight loss and macroscopic and microscopic scores. TNBS-induced colitis significantly increased the colonic mRNA expression of tumor necrosis factor-alpha (TNF-?), interleukin-1beta (IL-1ß), and caspase-3 activities in group II in comparison to the group I. HNG treatment was associated with an inhibition of mRNA expression of TNF-? and IL-1ß, and a decrease in caspase-3 activities in colon tissues in group III and IV when compared to group II. Conclusion: The results of this study indicate that HNG treatment may exert beneficial effects in UC by decreasing inflammatory reactions and apoptosis. © 2017 Saudi Journal of Gastroenterology (Official journal of The Saudi Gastroenterology Association) | Published by Wolters Kluwer - Medknow. |
| Databáze: | OpenAIRE |
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