Dental color measurement to predict DNA concentration in incinerated teeth for human identification
| Autor: | Maria Jesus Gaitan, Jose Manuel Sioli, Leticia Rubio, Stella Martin-de-las-Heras |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
Teeth Hot Temperature Time Factors Light Physiology Digestive Physiology lcsh:Medicine Social Sciences Artificial Gene Amplification and Extension Incineration Polymerase Chain Reaction 01 natural sciences chemistry.chemical_compound Spectrum Analysis Techniques 0302 clinical medicine Spectrophotometry Medicine and Health Sciences lcsh:Science Colorimetry DNA extraction Multidisciplinary medicine.diagnostic_test Physics Electromagnetic Radiation Dna concentration Middle Aged Clinical Laboratory Sciences Real-time polymerase chain reaction Physical Sciences Significant positive correlation Female Chloroform Anatomy Research Article Adult Visible Light Color Fires 03 medical and health sciences Extraction techniques Diagnostic Medicine medicine Dentition Humans Color measurement 030216 legal & forensic medicine Chromaticity Molecular Biology Techniques Molecular Biology Forensics Chromatography Phenol lcsh:R 010401 analytical chemistry Biology and Life Sciences DNA 0104 chemical sciences Research and analysis methods Luminance Jaw chemistry Dentin Forensic Anthropology lcsh:Q Law and Legal Sciences Digestive System Head Tooth |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname PLoS ONE PLoS ONE, Vol 13, Iss 4, p e0196305 (2018) |
| Popis: | Teeth exposed to thermal stress can shed light on the identification of incinerated individuals and on the circumstances of the fire. Changes in the color of burned teeth can provide information on structural changes and the temperature of exposure. The objective of this study was to correlate color modifications with the concentration of human DNA in teeth burned at different temperatures. Spectrophotometry was used to measure the color of 40 teeth heated at temperatures of 100, 200, and 400ÊC for 60 min. DNA was extracted by phenol-chloroform extraction and quantified by real-time quantitative PCR using the Quantifier human DNA quantification kit. Preliminary results indicated an association of higher temperature with changes in colorimetric variables and a decrease in DNA concentrations. A significant positive correlation was found between luminosity values and DNA concentration (r = 0.4727, p = 0.0128) and between chromaticity a* values and DNA concentration (r = 0.4154, p = 0.0250). Spectrophotometry analysis of the color of burned teeth may predict the feasibility of extracting human DNA for identification purposes. This work was supported by the University of Malaga (PPIT.UMA.B1.2017/24). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| Databáze: | OpenAIRE |
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