Follicular flushing leads to higher oocyte yield in monofollicular IVF: a randomized controlled trial
| Autor: | Marie C. Roumet, A Kohl Schwartz, S Weidlinger, A Wueest, Brigitte Leeners, I Calzaferri, A Fink, V Mitter, M. von Wolff, Andreas Limacher |
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| Přispěvatelé: | University of Zurich, Kohl Schwartz, A S |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pregnancy Rate
Oocyte Retrieval law.invention 0302 clinical medicine Randomized controlled trial law Pregnancy Follicular phase Obstetrics and Gynaecology 030212 general & internal medicine Prospective Studies 610 Medicine & health Prospective cohort study 030219 obstetrics & reproductive medicine Rehabilitation Obstetrics and Gynecology 10175 Clinic for Reproductive Endocrinology IVF follicular flushing Female medicine.symptom Live birth Infertility Female Switzerland Infertility medicine.medical_specialty Fertilization in Vitro 03 medical and health sciences medicine Flushing Humans monofollicular IVF Retrospective Studies Gynecology single-lumen needle business.industry oocyte yield Retrospective cohort study 2729 Obstetrics and Gynecology Original Articles 2743 Reproductive Medicine medicine.disease AcademicSubjects/MED00905 live birth rate Pregnancy rate Reproductive Medicine Oocytes business |
| Zdroj: | Human Reproduction (Oxford, England) Kohl Schwartz, A. S.; Calzaferri, I; Roumet, M.; Limacher, A.; Fink, A.; Wueest, A; Weidlinger, S.; Mitter, V.R.; Leeners, B; Von Wolff, M. (2020). Follicular flushing leads to higher oocyte yield in monofollicular IVF: a randomized controlled trial. Human reproduction, 35(10), pp. 2253-2261. Oxford University Press 10.1093/humrep/deaa165 |
| DOI: | 10.1093/humrep/deaa165 |
| Popis: | STUDY QUESTION Does follicular flushing increase the number of mature oocytes in monofollicular IVF? SUMMARY ANSWER Follicular flushing increases the number of mature oocytes in monofollicular IVF. WHAT IS KNOWN ALREADY Flushing increases neither the oocyte yield nor the pregnancy rate in polyfollicular IVF or in poor responder patients. In monofollicular IVF, the effect of flushing has so far been addressed by two studies: (i) a prospective study with minimal stimulation IVF demonstrated an increased oocyte yield, and (ii) a retrospective study with natural cycle (NC)-IVF showed an increased oocyte yield and an increased transfer rate. STUDY DESIGN, SIZE, DURATION Randomized controlled trial including 164 women who were randomized for either aspiration with or without flushing from 2016 to 2019. PARTICIPANTS/MATERIALS, SETTING, METHODS Infertile women 18–42 years of age with an indication for IVF treatment at a university-based infertility unit. Women undergoing monofollicular IVF were randomized to either follicular aspiration only or follicular aspiration directly followed by five follicular flushes at a 1:1 ratio. The intervention was done without anaesthesia, using a gauge 19 single-lumen needle. Flushing volume was calculated (sphere formula) based on the size of the follicle. MAIN RESULTS AND THE ROLE OF CHANCE A total of 164 women were included; 81 were allocated to ‘aspiration only’ and 83 to additional ‘flushing’. Primary analysis was based on the intention-to-treat: oocyte yield, defined as the collected mature oocyte rate, was higher (n = 64/83, 77.1%) in the flushing group compared to the aspiration only group (n = 48/81, 59.3%, adjusted risk difference (RD): 18.2% (95% CI 3.9–31.7%), P-value = 0.02). In the flushing group, most oocytes were retrieved within the first three flushes (63/83, 75.8%). Fertilization rate was higher in the flushing group (n = 53/83, 63.9% vs n = 38/81, 46.9%; adjusted RD: 16.8% (96% CI 1.5–31.4%), P = 0.045). Transfer rate was also higher in the flushing group (n = 52/83, 62.7% vs n = 38/81, 46.9%; RD: 15.71 (95% CI 0.3–30.3%)), but the difference was not significant (P = 0.06). The clinical pregnancy rate n = 9/83 versus n = 9/81 (RD: −0.3% (95% CI −9.9% to 9.5%)) and live birth rate n = 7/83 versus n = 8/81 (RD: −1.5% (95% CI −10.4% to 7.1%)) were not significantly different between the flushing and the aspiration group. The median duration of the intervention was significantly longer with flushing (2.38 min; quartiles 2.0, 2.7) versus aspiration only (0.43 min; quartiles 0.3, 0.5) (P LIMITATIONS, REASONS FOR CAUTION Blinding of the procedure was not possible. WIDER IMPLICATIONS OF THE FINDINGS Our study proved that flushing of single follicles in NC-IVF increases the oocyte yield. In contrast to polyfollicular IVF flushing seems to be beneficial in a monofollicular setting if the technique used in our study (single-lumen needle, 5 flushings with flushing volume adaptation) is applied. STUDY FUNDING/COMPETING INTEREST(S) The study was funded by the financial sources of the division and in part by a research grant provided by NMS Biomedical SA, Switzerland. The company did not have any roles in design or conduct of the study or in the preparation of the manuscript. The authors have no other conflicts of interest. TRIAL REGISTRATION NUMBER Clinicaltrials.gov NCT 02641808. TRIAL REGISTRATION DATE 29 December 2015 DATE OF FIRST PATIENT’S ENROLMENT 22 August 2016 |
| Databáze: | OpenAIRE |
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