Molecular and immunological evaluation of the transcription factor SOX-4 as a lung tumor vaccine antigen

Autor: Rachel S. Friedman, Chaitanya S. Bangur, Liqun Fan, Michael Kalos, Eden J. Zasloff, Yoshihiro Watanabe, Tongtong Wang
Rok vydání: 2004
Předmět:
CD4-Positive T-Lymphocytes
endocrine system
Lung Neoplasms
Antibodies
Neoplasm
T cell
Immunology
Antigen presentation
Molecular Sequence Data
Drug Evaluation
Preclinical
Epitopes
T-Lymphocyte
CD8-Positive T-Lymphocytes
Lymphocyte Activation
Cancer Vaccines
Polymerase Chain Reaction
Epitope
SOXC Transcription Factors
Immune system
Antibody Specificity
Carcinoma
Non-Small-Cell Lung
Cell Line
Tumor
medicine
Immunology and Allergy
Cytotoxic T cell
Humans
Amino Acid Sequence
Carcinoma
Small Cell
Lung cancer
Cells
Cultured
Cell Line
Transformed
Antigen Presentation
biology
urogenital system
Large cell
High Mobility Group Proteins
medicine.disease
Peptide Fragments
respiratory tract diseases
Neoplasm Proteins
medicine.anatomical_structure
embryonic structures
biology.protein
Leukocytes
Mononuclear
Trans-Activators
Antibody
Zdroj: Journal of immunology (Baltimore, Md. : 1950). 172(5)
ISSN: 0022-1767
Popis: The developmental transcription factor SOX-4 has been shown to be highly and differentially overexpressed in primary small cell lung carcinomas (SCLC). To examine the potential of SOX-4 for broad use as a lung cancer vaccine, we have evaluated the expression of SOX-4 in a panel of primary adenocarcinoma, squamous, and large cell tumor samples as well as in a panel of established small cell and non-small cell lung carcinoma tumor cell lines. SOX-4 mRNA is shown to be overexpressed in a substantial fraction of each of these lung tumor types. To examine the immunological potential of SOX-4, we have evaluated the presence of SOX-4-specific CD4 and CD8 T cells in PBMC of healthy donors and the presence of SOX4-specific Abs in sera from SCLC patients. We demonstrate the presence of both CD4 and CD8 T cells that recognize naturally processed epitopes derived from SOX-4 as well as the presence of SOX-4-specific Abs in sera from SCLC patients, but not in sera from healthy donors. The lung tumor-specific overexpression and demonstration of a comprehensive Ag-specific immune response specific for SOX-4 support the use of this molecule in the development of whole gene-, peptide-, or protein-based vaccination strategies against lung cancer. Furthermore, the identification of naturally processed T cell and Ab epitopes from SOX-4 provides valuable tools for the development of peptide-based vaccination strategies against lung cancer as well as to monitor SOX-4-specific responses in vaccinated patients.
Databáze: OpenAIRE
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