Changes in the binding characteristics of the μ, δ and κ subtypes of the opioid receptor in the hypothalamus of the normal cyclic ewe and in the ovariectomised ewe following treatment with ovarian steroids
| Autor: | P. J. Wright, B J Canny, Iain J. Clarke, Michael A. Cowley, B Thom |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Ovariectomy Endocrinology Diabetes and Metabolism medicine.medical_treatment Hypothalamus Receptors Opioid mu Diprenorphine Luteal phase Biology δ-opioid receptor Endocrinology Estrus Opioid receptor Receptors Opioid delta Internal medicine Follicular phase medicine Animals Opioid peptide Receptor Progesterone Sheep Estradiol Receptors Opioid kappa Luteinizing Hormone Steroid hormone Receptors Opioid Female Protein Binding medicine.drug |
| Zdroj: | Journal of Endocrinology. 149:509-518 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1677/joe.0.1490509 |
| Popis: | The μ, δ and κ opioid receptor subtypes were measured across the oestrous cycle of the ewe and in ovariectomised (OVX) ewes treated with oestrogen and/or progesterone. We have used a subtype-specific opioid receptor binding assay, in which [3H]diprenorphine non-preferentially labelled each receptor subtype in the presence of blocking concentrations of site-specific opioid analogues. The density and affinity of each receptor subtype was measured in the preoptic area (POA) of the hypothalamus and the mediobasal hypothalamus (MBH). Normally cycling ewes were killed during the luteal phase of the oestrous cycle and at various times after an injection of a synthetic prostaglandin (cloprostenol) to synchronise the onset of the follicular phase. OVX ewes were either untreated as controls (n=4) or treated with oestrogen (n=4), progesterone (n=4) or oestrogen and progesterone combined (n=4). The total number of opioid receptors did not alter across the oestrous cycle or with steroid hormone treatment. In the POA, the mean (± s.e.m.) number of δ receptors was significantly (PPPPPP Journal of Endocrinology (1996) 149, 509–518 |
| Databáze: | OpenAIRE |
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