Coagulation measurement from whole blood using vibrating optical fiber in a disposable cartridge
| Autor: | Yusuf Samet Yaras, Hakan Urey, Ibrahim Baris, Gokhan Saglam, Selim Olcer, Fehmi Civitci, Ali Bars Gündüz, Göksenin Yaralıoğlu |
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| Přispěvatelé: | Özyeğin University, Yaralıoğlu, Göksenin, Yaras, Yusuf Samet, Gündüz, Ali Bars, Sağlam, Gökhan, Ölçer, Selim, Ürey, Hakan, Çivitci, Fehmi, Barış, İbrahim, Optical Microsystems Laboratory (OML), College of Engineering, Department of Electrical and Electronics Engineering |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Materials science
Optical fiber Point-of-Care Systems Microfluidics Biomedical Engineering 030204 cardiovascular system & hematology 01 natural sciences law.invention Biomaterials 03 medical and health sciences Cartridge 0302 clinical medicine law Lab-On-A-Chip Devices Humans Mechanical resonance Fiber Blood Coagulation Optical Fibers Whole blood Fiber optic sensor Blood coagulation Activated partial thromboplastin time Point-of-care Lab-on-a-chip Radiology Nuclear medicine & medical imaging Hematologic Tests 010401 analytical chemistry Atomic and Molecular Physics and Optics 0104 chemical sciences Electronic Optical and Magnetic Materials Biochemistry and molecular biology Optics Biomedical engineering |
| Zdroj: | Journal of Biomedical Optics |
| DOI: | 10.1117/1.JBO.22.11.117001.full |
| Popis: | In clinics, blood coagulation time measurements are performed using mechanical measurements with blood plasma. Such measurements are challenging to do in a lab-on-a-chip (LoC) system using a small volume of whole blood. Existing LoC systems use indirect measurement principles employing optical or electrochemical methods. We developed an LoC system using mechanical measurements with a small volume of whole blood without requiring sample preparation. The measurement is performed in a microfluidic channel where two fibers are placed inline with a small gap in between. The first fiber operates near its mechanical resonance using remote magnetic actuation and immersed in the sample. The second fiber is a pick-up fiber acting as an optical sensor. The microfluidic channel is engineered innovatively such that the blood does not block the gap between the vibrating fiber and the pick-up fiber, resulting in high signal-to-noise ratio optical output. The control plasma test results matched well with the plasma manufacturer's datasheet. Activated-partial-thromboplastin-time tests were successfully performed also with human whole blood samples, and the method is proven to be effective. Simplicity of the cartridge design and cost of readily available materials enable a low-cost point-of-care device for blood coagulation measurements. Scientific and Technological Research Council of Turkey (TÜBİTAK) |
| Databáze: | OpenAIRE |
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