First biocompatibility margins for optical stimulation at the eardrum via 532-nm laser pulses in a mouse model
| Autor: | Hans-Jochen Foth, Katharina Sorg, Lukas Pillong, Patricia Stahn, Marius Hinsberger, Gentiana I. Wenzel, Larissa Heimann, Bernhard Schick |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Paper
Optics and Photonics Tympanic Membrane Materials science Light Biocompatibility Laser safety Biomedical Engineering Ear Middle Apoptosis Biocompatible Materials Stimulation optical stimulation 01 natural sciences law.invention Photoacoustic Techniques 010309 optics Biomaterials Mice Necrosis biocompatibility Hearing Aids Bone conduction Hearing law 0103 physical sciences Evoked Potentials Auditory Brain Stem otorhinolaryngologic diseases medicine Animals General Lasers Temperature Laser optoacoustic Atomic and Molecular Physics and Optics laser Electronic Optical and Magnetic Materials Electrophysiology medicine.anatomical_structure Microscopy Fluorescence Optical stimulation Mice Inbred CBA Middle ear Blood Vessels Female Eardrum Photic Stimulation Biomedical engineering |
| Zdroj: | Journal of Biomedical Optics |
| ISSN: | 1083-3668 |
| Popis: | Hearing impairment affects ∼460 million people worldwide. Conservative therapies, such as hearing aids, bone conduction systems, and middle ear implants, do not always sufficiently compensate for this deficit. The optical stimulation is currently under investigation as an alternative stimulation strategy for the activation of the hearing system. To assess the biocompatibility margins of this emerging technology, we established a method applicable in whole-mount preparations of murine tympanic membranes (TM). We irradiated the TM of anesthetized mice with 532-nm laser pulses at an average power of 50, 89, 99, and 125 mW at two different locations of the TM and monitored the hearing function with auditory brainstem responses. Laser-power-dependent negative side effects to the TM were observed at power levels exceeding 89 mW. Although we did not find any significant negative effects of optical stimulation on the hearing function in these mice, based on the histology results further studies are necessary for optimization of the used parameters. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|