Hepatitis E virus replication in human intestinal cells
| Autor: | Sébastien Lhomme, Olivier Marion, Nicolas Carrere, Florence Abravanel, Marion Migueres, Nassim Kamar, Mélanie Pucelle, Pierre Delobel, Martine Dubois, Bertrand Suc, Mary Requena, Manon Nayrac, Jean-Marie Peron, Jacques Izopet |
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| Přispěvatelé: | Service de Néphrologie et transplantation d'organes CHU Rangueil TOULOUSE, Centre de Physiopathologie Toulouse Purpan (CPTP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Virologie [Toulouse], CHU Toulouse [Toulouse], Service Hepato-Gastro-Enterologie, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse], Université Fédérale Toulouse Midi-Pyrénées, Service de chirurgie digestive, Service des maladies infectieuses et tropicales [Toulouse], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse] |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
viruses medicine.disease_cause Virus Replication MESH: Genotype chemistry.chemical_compound 0302 clinical medicine Hepatitis E virus [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Genotype Intestine Small MESH: Hepatocytes / drug effects Cells Cultured Gastroenterology virus diseases Hepatitis E 3. Good health MESH: Virus Replication / genetics medicine.anatomical_structure MESH: Epithelial Cells RNA Viral 030211 gastroenterology & hepatology MESH: Cells Cultured Biology Sensitivity and Specificity 03 medical and health sciences MESH: Intestine Small / cytology Antigen Ribavirin medicine Humans MESH: RNA Viral / genetics Differential centrifugation MESH: Humans enteric infections Epithelial Cells MESH: Hepatitis E virus / genetics medicine.disease Virology MESH: Sensitivity and Specificity digestive system diseases Small intestine MESH: Hepatocytes / virology 030104 developmental biology chemistry MESH: Ribavirin / pharmacology Hepatocytes antiviral chemotherapy hepatitis E Explant culture |
| Zdroj: | Gut Gut, BMJ Publishing Group, 2020, 69 (5), pp.901-910. ⟨10.1136/gutjnl-2019-319004⟩ |
| ISSN: | 1468-3288 0017-5749 |
| DOI: | 10.1136/gutjnl-2019-319004⟩ |
| Popis: | ObjectiveHepatitis E virus (HEV), one of the most common agent of acute hepatitis worldwide, is mainly transmitted enterically, via contaminated water for HEV genotypes 1 (HEV1) and HEV2, or by eating raw or undercooked infected meat for HEV genotype 3 (HEV3) and HEV4. However, little is known about how the ingested HEV reaches the liver or its ability to replicate in intestinal cells.DesignWe developed human primary cultures of small intestine epithelial cells and intestinal explants obtained from small bowel resections. The epithelial cells were also polarised on transwells. Cells were infected with Kernow-p6 strain or clinically derived virions.ResultsPrimary intestinal cells supported the growth of Kernow-p6 strain and HEV1 and HEV3 clinically derived virions. Polarised enterocytes infected with HEV1 and HEV3 strains released HEV particles vectorially: mostly into the apical compartment with a little basally. Iodixanol density gradient centrifugation of enterocyte-derived HEV virions gave bands at a density of 1.06–1.08 g/cm3, corresponding to that of quasi-enveloped HEV particles. Ribavirin therapy inhibited HEV excretion from the basal surface but not from the apical side of infected human enterocytes. HEV virions also infected intestinal tissue explants. Lastly, HEV RNA and antigen were detected in the intestinal crypts of a chronically infected patient.ConclusionHEV can replicate in intestinal cells and reaches the liver as quasi-enveloped virions. |
| Databáze: | OpenAIRE |
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