Localization of estrogen receptor in the central lymphoid organs of chickens during the late stage of embryogenesis
| Autor: | Yasuhiro Kondo, Kiyoaki Narabara, Asaki Abe, Tomokazu Fukuda, Masafumi Katayama |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
animal structures medicine.drug_class Lymphocyte Estrogen receptor Embryonic Development Chick Embryo Thymus Gland Biology Applied Microbiology and Biotechnology Biochemistry Hassall's corpuscles Analytical Chemistry Immune system Bursa of Fabricius Internal medicine medicine Animals Lymphocytes RNA Messenger Molecular Biology Organic Chemistry Gene Expression Regulation Developmental General Medicine Epithelium Cell biology Protein Transport medicine.anatomical_structure Lymphatic system Endocrinology Receptors Estrogen Estrogen Organ Specificity embryonic structures Estrogen receptor alpha Chickens hormones hormone substitutes and hormone antagonists Spleen Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 76(11) |
| ISSN: | 1347-6947 |
| Popis: | Immunological function in chicks is greatly affected by estrogen treatment during embryogenesis, but the mechanism of the estrogen effect is not fully understood. To elucidate the effect of estrogen on immune function, we observed estrogen receptor expression in the thymus and bursa of chick embryos by immunohistochemistry. We compared the distribution of estrogen receptor-positive cells with that of keratin-positive epithelial cells. Intense expression of estrogen receptors was detected in thymic and bursal lymphocytes. In peripheral lymphocytes, ER mRNA was detected by RT-PCR analysis. The results of fluorescence-activated cell sorting analysis indicated that the estrogen receptor was expressed in the cytoplasm of the lymphocytes. Furthermore, intense expression of the estrogen receptor was also confirmed in thymic Hassall's corpuscles, bursal follicle-associated epithelial cells, and the bursal interfollicular epithelium. Our results indicate that estrogen affects the differentiation of thymic and bursal lymphocytes, suggesting that the underlying role for estrogen in immune function. |
| Databáze: | OpenAIRE |
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