Diagnostic Utility of IMP3 Expression in Thyroid Neoplasms
| Autor: | Christopher W. Roth, Andre M. Oliveira, Ricardo V. Lloyd, Shuya Zhang, Long Jin, Michele R. Erickson-Johnson, Barbara R. Evers, Amber R. Seys |
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| Rok vydání: | 2010 |
| Předmět: |
Adenoma
Male endocrine system Pathology medicine.medical_specialty endocrine system diseases medicine.medical_treatment Sensitivity and Specificity Pathology and Forensic Medicine Thyroid carcinoma Follicular phase medicine Humans RNA Messenger Thyroid Neoplasms Pathology Molecular Molecular Biology Hyperplasia Reverse Transcriptase Polymerase Chain Reaction business.industry Growth factor Carcinoma Thyroid RNA-Binding Proteins RNA Cell Biology Middle Aged Fold change Real-time polymerase chain reaction medicine.anatomical_structure Immunohistochemistry Female business |
| Zdroj: | Diagnostic Molecular Pathology. 19:63-69 |
| ISSN: | 1052-9551 |
| Popis: | The capability of molecular markers to differentiate between benign and malignant well-differentiated thyroid tumors remains unclear. The aim of this study was to evaluate the use of insulin-like growth factor II mRNA binding protein-3 (IMP3) mRNA expression to distinguish benign from malignant thyroid tumors. RNA samples from 80 formalin-fixed, paraffin-embedded thyroid tissues, including 22 usual papillary thyroid carcinomas (PTCs), 18 follicular variants of PTC, 5 follicular thyroid carcinomas, 33 follicular adenomas, and 2 hyperplastic nodules, were used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. IMP3 mRNA expression levels in thyroid tumors were expressed as relative fold change (fold) after normalization with normal thyroid RNA. The results showed that thyroid carcinomas including PTC, follicular variants of PTC, and follicular thyroid carcinomas have significantly higher IMP3 expression levels with 48.3, 35.3, and 43.8 fold, respectively, compared with benign thyroid lesions (2.8 fold). Using the IMP3 expression value of 5 fold as a cutoff point to separate benign and malignant thyroid tumors, IMP3 qRT-PCR analysis had a 91.4% clinical specificity and 86.7% clinical sensitivity for the diagnosis of well-differentiated thyroid carcinomas. Conventional RT-PCR and immunohistochemical analysis for IMP3 in a subset of cases supported the qRT-PCR results. These results indicate that detection of IMP3 mRNA expression levels by qRT-PCR may be a useful molecular marker to assist in the diagnosis of well-differentiated thyroid carcinomas. |
| Databáze: | OpenAIRE |
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