Ascorbate-2-phosphate inhibition of ascorbate-2-sulfate sulfohydrolase from bovine liver

Autor: Mancourt Downing, Paul A. Seib, Russell W. Carlson, Bert M. Tolbert
Rok vydání: 1976
Předmět:
Zdroj: Biochemical and Biophysical Research Communications. 68:949-953
ISSN: 0006-291X
Popis: The hydrolysis of ascorbate-2-sulfate by the enzyme, ascorbate-2-sulfate sulfohydrolase, purified from bovine liver has been shown to be powerfully inhibited by ascorbate-2-phosphate. The inhibition by ascorbate phosphate is competitive with a KI of 0.3 μM. Na2HPO4 also inhibits by an apparent non-competitive process. The Na2HPO4 concentration at 50% inhibition is 7.7 μM. A possible control role for ascorbate phosphate in ascorbate biochemistry is suggested.
Databáze: OpenAIRE