Ascorbate-2-phosphate inhibition of ascorbate-2-sulfate sulfohydrolase from bovine liver
| Autor: | Mancourt Downing, Paul A. Seib, Russell W. Carlson, Bert M. Tolbert |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
chemistry.chemical_classification
Binding Sites Chemistry Biophysics Ascorbic Acid Cell Biology Phosphate Binding Competitive Biochemistry Phosphates Kinetics chemistry.chemical_compound Hydrolysis Organophosphorus Compounds Ascorbate 2-sulfate Enzyme Liver Animals Cattle Sulfatases Molecular Biology Arylsulfatases Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 68:949-953 |
| ISSN: | 0006-291X |
| Popis: | The hydrolysis of ascorbate-2-sulfate by the enzyme, ascorbate-2-sulfate sulfohydrolase, purified from bovine liver has been shown to be powerfully inhibited by ascorbate-2-phosphate. The inhibition by ascorbate phosphate is competitive with a KI of 0.3 μM. Na2HPO4 also inhibits by an apparent non-competitive process. The Na2HPO4 concentration at 50% inhibition is 7.7 μM. A possible control role for ascorbate phosphate in ascorbate biochemistry is suggested. |
| Databáze: | OpenAIRE |
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