A Systematic Study of RNAi Effects and dsRNA Stability in Tribolium castaneum and Acyrthosiphon pisum, Following Injection and Ingestion of Analogous dsRNAs

Autor: Min Cao, Elaine Fitches, John A. Gatehouse
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
animal structures
RNA Stability
Gene Expression
pea aphid (Acyrthosiphon pisum)
Catalysis
Article
Pisum
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
exo-nucleases
Eating
RNA interference
flour beetle (Tribolium castaneum)
Hemolymph
Gene expression
Animals
Red flour beetle
Physical and Theoretical Chemistry
Molecular Biology
Gene
lcsh:QH301-705.5
Spectroscopy
RNA
Double-Stranded
dsRNA stability
Tribolium
RNAi
endo-nucleases
biology
Organic Chemistry
fungi
food and beverages
Gene Expression Regulation
Developmental
General Medicine
biology.organism_classification
Molecular biology
Animal Feed
Computer Science Applications
Acyrthosiphon pisum
RNA silencing
030104 developmental biology
Phenotype
lcsh:Biology (General)
lcsh:QD1-999
Aphids
Insect Proteins
RNA Interference
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 19, Iss 4, p 1079 (2018)
International Journal of Molecular Sciences; Volume 19; Issue 4; Pages: 1079
International journal of molecular sciences, 2018, Vol.19(4), pp.1079 [Peer Reviewed Journal]
ISSN: 1422-0067
Popis: RNA interference (RNAi) effects in insects are highly variable and may be largely dependent upon the stability of introduced double-stranded RNAs to digestion by nucleases. Here, we report a systematic comparison of RNAi effects in susceptible red flour beetle (Tribolium castaneum) and recalcitrant pea aphid (Acyrthosiphon pisum) following delivery of dsRNAs of identical length targeting expression of V-type ATPase subunit E (VTE) and inhibitor of apoptosis (IAP) genes. Injection and ingestion of VTE and IAP dsRNAs resulted in up to 100% mortality of T. castaneum larvae and sustained suppression (>80%) of transcript levels. In A. pisum, injection of VTE but not IAP dsRNA resulted in up to 65% mortality and transient suppression (ca. 40%) of VTE transcript levels. Feeding aphids on VTE dsRNA reduced growth and fecundity although no evidence for gene suppression was obtained. Rapid degradation of dsRNAs by aphid salivary, haemolymph and gut nucleases contrasted with stability in T. castaneum larvae where it appears that exo-nuclease activity is responsible for relatively slow digestion of dsRNAs. This is the first study to directly compare RNAi effects and dsRNA stability in receptive and refractory insect species and provides further evidence that dsRNA susceptibility to nucleases is a key factor in determining RNAi efficiency.
Databáze: OpenAIRE
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