A simple integrated microfluidic device for the multiplexed fluorescence-free detection of Salmonella enterica
| Autor: | Daniel Nelson, Jacob C. Lee, Hillary S. Sloane, Eric R. Houpt, Daniel C. Miranian, James P. Landers, Briony C. Strachan, Jingyi Li |
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| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial Materials science Microfluidics Nanotechnology 02 engineering and technology Nucleic Acid Testing 01 natural sciences Biochemistry Multiplexing Polymerase Chain Reaction Article Analytical Chemistry Lab-On-A-Chip Devices Electrochemistry Pressure Environmental Chemistry Polymethyl Methacrylate Fluidics Spectroscopy biology 010401 analytical chemistry Nucleic Acid Hybridization Salmonella enterica 021001 nanoscience & nanotechnology biology.organism_classification Fluorescence 0104 chemical sciences Systems Integration Torque Nat Magnetic nanoparticles 0210 nano-technology Biomedical engineering |
| Zdroj: | The Analyst. 141(3) |
| ISSN: | 1364-5528 |
| Popis: | Rapid, inexpensive and simplistic nucleic acid testing (NAT) is pivotal in delivering biotechnology solutions at the point-of-care (POC). We present a poly(methylmethacrylate) (PMMA) microdevice where on-board infrared-mediated PCR amplification is seamlessly integrated with a particle-based, visual DNA detection for specific detection of bacterial targets in less than 35 minutes. Fluidic control is achieved using a capillary burst valve laser-ablated in a novel manner to confine the PCR reagents to a chamber during thermal cycling, and a manual torque-actuated pressure system to mobilize the fluid from the PCR chamber to the detection reservoir containing oligonucleotide-adducted magnetic particles. Interaction of amplified products specific to the target organism with the beads in a rotating magnetic field allows for near instantaneous ( |
| Databáze: | OpenAIRE |
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