Detection of Human T Lymphotropic Virus Type II/b in Human Immunodeficiency Virus Type 1-Coinfected Persons in Southeastern Italy
| Autor: | Josè Ramòn Fiore, Monica Luparello, Gioacchino Angarano, Annarosa Del Mistro, Antonella Grottola, L. Chieco Bianchi, Maria Luisa Calabrò |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
viruses
Molecular Sequence Data Restriction Mapping Human T-lymphotropic virus Virus Replication Genes env Polymerase Chain Reaction Virus law.invention Serology Immunoenzyme Techniques Risk Factors immune system diseases law hemic and lymphatic diseases HIV Seropositivity Humans Immunology and Allergy Serotyping Polymerase chain reaction Base Sequence biology Viral culture Human T-lymphotropic virus 2 virus diseases biology.organism_classification Virology Infectious Diseases Italy HTLV-II Infections HIV-1 Viral disease |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/168.5.1273 |
| Popis: | A preliminary screening of 511 persons at risk for AIDS living in southeastern Italy disclosed 20 cases of seroreactivity to human T lymphotropic viruses (HTLV). To verify and type the HTLV infection among these subjects, confirmatory serologic tests, polymerase chain reaction (PCR), and virus culture were done. No evidence of HTLV-I infection was found. HTLV-II infection was confirmed in 8 cases by HTLV-specific, synthetic peptide EIAs and PCR on uncultured cells; restriction analysis of the PCR-amplified env regions revealed the presence of HTLV-II/b strains in all 8 cases. Four sera were nontypeable by EIA. The finding of such indeterminate reactivities in a geographic area in which HTLV variants were previously described indicates the need for more extensive surveys among the healthy population. HTLV-II was isolated in 5 cases, and virus isolation was mostly dependent on the presence of an actively replicating human immunodeficiency virus type 1 in culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|