The TPLATE complex mediates membrane bending during plant clathrin–mediated endocytosis

Autor: Alexander Johnson, Dana A. Dahhan, Nataliia Gnyliukh, Walter A. Kaufmann, Vanessa Zheden, Tommaso Costanzo, Pierre Mahou, Mónika Hrtyan, Jie Wang, Juan Aguilera-Servin, Daniël Van Damme, Emmanuel Beaurepaire, Martin Loose, Sebastian Y. Bednarek, Jiří Friml
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Proceedings of the National Academy of Sciences of the United States of America
Proc Natl Acad Sci U S A
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN: 1091-6490
0027-8424
Popis: Significance Endocytosis transports cargos inside the cell by creating spherical vesicles from the plasma membrane. This membrane remodeling requires proteins to generate force to bend the membrane inwards, overcoming the high-turgor pressure in plant cells. However, as plants create clathrin-coated vesicles without actin, the machinery to bend membranes during endocytosis is entirely unknown and appears distinct from other model systems. Here, we refine the physiological role of the plant-specific and essential endocytic TPLATE complex. We find it localizes outside of clathrin-coated vesicles and mediates membrane bending, contrasting with previous predictions. We further demonstrate that the TPLATE complex contains protein domains which have intrinsic membrane-bending activity; thus, we identify a component of the unique endocytosis membrane-bending machinery in plants.
Clathrin-mediated endocytosis is the major route of entry of cargos into cells and thus underpins many physiological processes. During endocytosis, an area of flat membrane is remodeled by proteins to create a spherical vesicle against intracellular forces. The protein machinery which mediates this membrane bending in plants is unknown. However, it is known that plant endocytosis is actin independent, thus indicating that plants utilize a unique mechanism to mediate membrane bending against high-turgor pressure compared to other model systems. Here, we investigate the TPLATE complex, a plant-specific endocytosis protein complex. It has been thought to function as a classical adaptor functioning underneath the clathrin coat. However, by using biochemical and advanced live microscopy approaches, we found that TPLATE is peripherally associated with clathrin-coated vesicles and localizes at the rim of endocytosis events. As this localization is more fitting to the protein machinery involved in membrane bending during endocytosis, we examined cells in which the TPLATE complex was disrupted and found that the clathrin structures present as flat patches. This suggests a requirement of the TPLATE complex for membrane bending during plant clathrin–mediated endocytosis. Next, we used in vitro biophysical assays to confirm that the TPLATE complex possesses protein domains with intrinsic membrane remodeling activity. These results redefine the role of the TPLATE complex and implicate it as a key component of the evolutionarily distinct plant endocytosis mechanism, which mediates endocytic membrane bending against the high-turgor pressure in plant cells.
Databáze: OpenAIRE
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