Calcium signaling and copper toxicity in Saccharomyces cerevisiae cells
| Autor: | Ileana C. Farcasanu, Lavinia L. Ruta, Ioana Nicolau, Claudia V. Popa |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
inorganic chemicals
0301 basic medicine Saccharomyces cerevisiae Proteins Health Toxicology and Mutagenesis Saccharomyces cerevisiae Aequorin chemistry.chemical_element Oxidative phosphorylation Calcium Cell membrane 03 medical and health sciences Cytosol 0302 clinical medicine medicine Environmental Chemistry Calcium Signaling Calcium signaling biology Cell Membrane Hydrogen Peroxide General Medicine biology.organism_classification Pollution 030104 developmental biology medicine.anatomical_structure chemistry Biochemistry biology.protein Biophysics Plasma membrane Ca2+ ATPase Copper 030217 neurology & neurosurgery |
| Zdroj: | Environmental Science and Pollution Research. 23:24514-24526 |
| ISSN: | 1614-7499 0944-1344 |
| DOI: | 10.1007/s11356-016-6666-5 |
| Popis: | To respond to metal surpluses, cells have developed intricate ways of defense against the excessive metallic ions. To understand the ways in which cells sense the presence of toxic concentration in the environment, the role of Ca2+ in mediating the cell response to high Cu2+ was investigated in Saccharomyces cerevisiae cells. It was found that the cell exposure to high Cu2+ was accompanied by elevations in cytosolic Ca2+ with patterns that were influenced not only by Cu2+ concentration but also by the oxidative state of the cell. When Ca2+ channel deletion mutants were used, it was revealed that the main contributor to the cytosolic Ca2+ pool under Cu2+ stress was the vacuolar Ca2+ channel, Yvc1, also activated by the Cch1-mediated Ca2+ influx. Using yeast mutants defective in the Cu2+ transport across the plasma membrane, it was found that the Cu2+-dependent Ca2+ elevation could correlate not only with the accumulated metal, but also with the overall oxidative status. Moreover, it was revealed that Cu2+ and H2O2 acted in synergy to induce Ca2+-mediated responses to external stress. |
| Databáze: | OpenAIRE |
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