Role of poly(ADP-ribose) polymerase activation in the pathogenesis of periodontitis in diabetes
| Autor: | Toshihide Noguchi, Tomokazu Saiki, Yasuko Kobayashi, Shin‐ichi Miyajima, Shuichiro Kobayashi, Makoto Mizutani, Megumi Miyabe, Norikazu Ohno, Tatsuaki Matsubara, Nobuhisa Nakamura, Akio Mitani, Keiko Naruse, Yuki Suzuki, Takeshi Kikuchi, Kei Adachi, Toru Nishikawa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Poly ADP ribose polymerase Gene Expression Poly(ADP-ribose) Polymerase Inhibitors Diabetes Mellitus Experimental Pathogenesis Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Diabetes mellitus medicine Animals Periodontitis Dental alveolus business.industry Monocyte 030206 dentistry X-Ray Microtomography medicine.disease Isoquinolines Rats 030104 developmental biology medicine.anatomical_structure PARP inhibitor Cancer research Periodontics Poly(ADP-ribose) Polymerases business Immunostaining |
| Zdroj: | Journal of clinical periodontology. 44(10) |
| ISSN: | 1600-051X |
| Popis: | Aim The aetiology of progressive periodontitis in diabetes has not yet been elucidated. We previously demonstrated that nitrosative stress is increased in diabetic rats with periodontitis. Nitrosative stress induces poly(ADP-ribose) polymerase (PARP) activation. Here, we demonstrated the involvement of PARP activation in diabetic periodontitis and detailed the therapeutic effects of PARP inhibitor. Materials and methods Experimental periodontitis was induced by placing a nylon thread ligature. Half of the normal and diabetic rats received the PARP inhibitor, 1,5-isoquinolinediol, for 2 weeks. Gingival PARP activation was detected by immunostaining for poly(ADP-ribose). Periodontitis was evaluated by gingival inflammatory cell infiltration, inflammatory gene expressions and micro-CT analyses. Results Although both periodontitis and the presence of diabetes increased PARP activation in the gingiva, diabetic rats with periodontitis had the highest activation of PARP. Diabetic rats with periodontitis also showed significant increases in monocyte/macrophage invasion into the gingiva, inflammatory gene expressions, nitrotyrosine-positive cells in the gingiva and alveolar bone loss, all of which were suppressed by treatment with the PARP inhibitor. Conclusions These results indicate the involvement of PARP activation in the pathogenesis and aggravation of periodontal disease in diabetes and suggest the therapeutic potential of PARP inhibition for treating periodontal disease, especially in patients with diabetes. |
| Databáze: | OpenAIRE |
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