Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy
| Autor: | Ehsan Gazi, Peter Gardner, Noel W. Clarke, T. J. Harvey, Richard D. Snook, Michael D Brown, Alex Henderson |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Cytoplasm diagnosis spectra Prostate cancer cell carcinoma Biochemistry Analytical Chemistry Prostate cancer Nuclear magnetic resonance Cell Line Tumor Manchester Institute of Biotechnology Spectroscopy Fourier Transform Infrared Electrochemistry medicine Carcinoma Humans Environmental Chemistry infrared micro-spectroscopy Fourier transform infrared spectroscopy ir microspectroscopy Spectroscopy Cell Nucleus Principal Component Analysis adenocarcinoma Chemistry Discriminant Analysis Prostatic Neoplasms Hyperplasia medicine.disease ResearchInstitutes_Networks_Beacons/manchester_institute_of_biotechnology Molecular biology Culture Media Prostate cell Cell culture Multivariate Analysis chemometric analysis raman-spectroscopy cytology Adenocarcinoma identification |
| Zdroj: | Harvey, T J, Gazi, E, Henderson, A, Snook, R D, Clarke, N W, Brown, M & Gardner, P 2009, ' Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy ', Analyst, vol. 134, no. 6, pp. 1083-1091 . https://doi.org/10.1039/b903249e |
| Popis: | In this study we obtained Fourier transform infrared (FTIR) spectra of fixed prostate cell lines of differing types as well as the primary epithelial cells from benign prostatic hyperplasia (BPH). Results showed that by using multivariate chemometric analysis it was possible to discriminate and classify these cell lines, which gave rise to sensitivity and specificity values of >94% and >98%, respectively. Following on from these results the possible influences of different factors on the discrimination and classification of the prostate cell lines were examined. Firstly, the effect of using different growth media during cell culturing was investigated, with results indicating that this did not influence chemometric discrimination. Secondly, differences in the nucleus-to-cytoplasm (N/C) ratio were examined, and it was concluded that this factor was not the main reason for the discrimination and classification of the prostate cancer (CaP) cell lines. In conclusion, given the fact that neither growth media nor N/C ratio could totally explain the classification it is likely that actual biochemical differences between the cell lines is the major contributing factor. © 2009 Royal Society of Chemistry. |
| Databáze: | OpenAIRE |
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