Pathway-focused gene expression profiles and immunohistochemistry detection identify contrasting association of caspase 3 (CASP3) expression with prognosis in pediatric classical Hodgkin lymphoma
| Autor: | Claudio Gustavo Stefanoff, Gerald Niedobitek, Priscilla Segges, Gabriela Vera-Lozada, Mário Henrique M. Barros, Rocio Hassan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Adolescent Disease-Free Survival 03 medical and health sciences 0302 clinical medicine Gene expression Humans RNA Messenger Reed-Sternberg Cells Child Gene Cyclin-dependent kinase 1 Tumor microenvironment biology Caspase 3 CENPF Hematology General Medicine Cell cycle Hodgkin Disease Immunohistochemistry 030104 developmental biology Oncology Tissue Array Analysis Apoptosis Child Preschool 030220 oncology & carcinogenesis Cancer research biology.protein Transcriptome |
| Zdroj: | Hematological Oncology. 36:663-670 |
| ISSN: | 0278-0232 |
| Popis: | The search for clinically relevant molecular markers in classical Hodgkin lymphoma (cHL) is hampered by the histopathological complexity of the disease, resulting from the admixture of a small number of neoplastic Hodgkin and Reed-Sternberg (H-RS) cells with an abundant and heterogeneous microenvironment. In this study, we evaluated gene expression profiles of 11 selected genes previously proposed as a molecular score for adult cHL, aiming to validate its application in the pediatric setting. Assays were performed by RT-qPCR from formalin-fixed paraffin-embedded (FFPE) lymph nodes in 80 patients with cHL. Selected genes were associated with cell cycle (CENPF, CDK1, CCNA2, CCNE2, and HMMR), apoptosis (BCL2, BCL2L1, and CASP3), and monocytes/macrophages (LYZ and STAT1). Despite using controlled preanalytical and analytical strategies, we were not able to validate the 11-gene score to be applied in pediatric cHL. Principal component analysis (PCA) disclosed 3 components that accounted for 65.7% of the total variability. The second PC included microenvironment and apoptosis genes, from which CASP3 expression was associated with a short time of progression-free survival, which impact was maintained in the unfavorable risk group, Epstein-Barr virus-negative cases, and multivariate analysis (P |
| Databáze: | OpenAIRE |
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