Binding of hairpin polyamides to DNA studied by fluorescence correlation spectroscopy for DNA nanoarchitectures
| Autor: | Partha P. Parui, Bernhard Brutschy, Chayan K. Nandi, Thorsten Schmidt, Alexander Heckel |
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| Rok vydání: | 2007 |
| Předmět: |
Molecular Structure
Base pair Chemistry Molecular Sequence Data Fluorescence spectrometry Fluorescence correlation spectroscopy DNA Biochemistry Single Molecule Spectroscopy Analytical Chemistry Nanostructures chemistry.chemical_compound Crystallography Nylons Spectrometry Fluorescence Polyamide Biophysics Molecule Linker |
| Zdroj: | Analytical and bioanalytical chemistry. 390(6) |
| ISSN: | 1618-2650 |
| Popis: | We have recently constructed a "DNA strut" consisting of two DNA-binding hairpin polyamides of Dervan-type connected via a long flexible linker and were able to show that this strut can be used to sequence-selectively connect DNA helices. This approach provides a second structural element (besides the Watson-Crick base pairing) for the assembly of higher-order DNA nanoarchitectures from smaller DNA building blocks. Since none of the existing analytical techniques for studying this kind of system were found suitable for detection and quantification of the formation of the resulting complexes, we chose fluorescence correlation spectroscopy (FCS). In the present study we show that FCS allowed us in a versatile and fast way to investigate the binding of Dervan polyamides to DNA. In particular it also shows its power in the quantitative detection of the formation of multimeric complexes and the in investigation of binding under nonphysiological conditions. |
| Databáze: | OpenAIRE |
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