Tryptase inhibits motility of human spermatozoa mainly by activation of the mitogen-activated protein kinase pathway
| Autor: | E. Wunn, R. Hollweck, Johannes Ring, Lars Kunz, Frank-Michael Köhn, M. Sbornik, Artur Mayerhofer, Stephan Weidinger, Martin Albrecht |
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| Rok vydání: | 2005 |
| Předmět: |
Male
MAPK/ERK pathway endocrine system MAP Kinase Signaling System Motility Tryptase In Vitro Techniques Pertussis toxin Second Messenger Systems Humans Receptor PAR-2 Phosphorylation reproductive and urinary physiology Sperm motility Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 biology urogenital system Serine Endopeptidases Rehabilitation Obstetrics and Gynecology Spermatozoa Sperm Cell biology Intracellular signal transduction Reproductive Medicine Mitogen-activated protein kinase Sperm Motility biology.protein Calcium Tryptases |
| Zdroj: | Human Reproduction. 20:456-461 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/humrep/deh618 |
| Popis: | BACKGROUND: We previously localized protease-activated receptor 2 (PAR-2) on human spermatozoa and demonstrated that activation of PAR-2 by the mast cell (MC) product tryptase inhibits sperm motility. Importantly, tryptase-secreting MCs are encountered in the male and female genital tract, implying that MC-spermatozoa interactions may be as yet unrecognized factors affecting sperm fertilizing ability. In order to elucidate how tryptase via activation of PAR-2 acts in human spermatozoa, we studied intracellular signal transduction events. METHODS AND RESULTS: Impairment of sperm motility by tryptase was not dependent on the presence of extracellular Ca 2+ and tryptase did not alter intracellular Ca 2+ levels. Pre-incubation with pertussis toxin (PTX) failed to prevent tryptase effects on sperm motility. Western blot analyses revealed that tryptase increased phosphorylation of the mitogen-activated protein kinases (MAPK) ERK1/2, an effect which was blocked by the MAPK pathway inhibitor PD98059. Pre-treatment of spermatozoa with this inhibitor also blocked the inhibtion of sperm motility evoked by tryptase. CONCLUSIONS: These results indicate that tryptase acts via the ERK1/2 pathway to inhibit motility of human spermatozoa. |
| Databáze: | OpenAIRE |
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