Screening of Chorioamnionitis Using Volatile Organic Compound Detection in Exhaled Breath: A Pre-clinical Proof of Concept Study
| Autor: | Daan R. M. G. Ophelders, Agnes W. Boots, Matthias C. Hütten, Salwan Al-Nasiry, Reint K. Jellema, Owen B. Spiller, Frederik-Jan van Schooten, Agnieszka Smolinska, Tim G. A. M. Wolfs |
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| Přispěvatelé: | RS: GROW - R4 - Reproductive and Perinatal Medicine, Kindergeneeskunde, Farmacologie en Toxicologie, RS: NUTRIM - R3 - Respiratory & Age-related Health, MUMC+: MA Medische Staf Kindergeneeskunde (9), Obstetrie & Gynaecologie, MUMC+: MA Medische Staf Obstetrie Gynaecologie (9), RS: MHeNs - R3 - Neuroscience |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
SPONTANEOUS PRETERM BIRTH Physiology Early detection BRAIN-INJURY exhaled breath volatile organic compound Chorioamnionitis Diagnostic tools DIAGNOSIS Pediatrics RJ1-570 NEUROINFLAMMATION 03 medical and health sciences 0302 clinical medicine INFLAMMATION medicine Risk factor OXIDATIVE STRESS Original Research CANDIDA-ALBICANS business.industry OVINE MODEL Clinical course Area under the curve preterm birth non-invasive diagnostics medicine.disease COMPOUNDS VOCS chorioamnionitis 030104 developmental biology Ureaplasma parvum Pediatrics Perinatology and Child Health Biomarker (medicine) biomarker business UREAPLASMA-PARVUM 030217 neurology & neurosurgery |
| Zdroj: | Frontiers in pediatrics, 9:617906. Frontiers Media S.A. Frontiers in Pediatrics Frontiers in Pediatrics, Vol 9 (2021) |
| ISSN: | 2296-2360 |
| DOI: | 10.3389/fped.2021.617906 |
| Popis: | Chorioamnionitis is a major risk factor for preterm birth and an independent risk factor for postnatal morbidity for which currently successful therapies are lacking. Emerging evidence indicates that the timing and duration of intra-amniotic infections are crucial determinants for the stage of developmental injury at birth. Insight into the dynamical changes of organ injury after the onset of chorioamnionitis revealed novel therapeutic windows of opportunity. Importantly, successful development and implementation of therapies in clinical care is currently impeded by a lack of diagnostic tools for early (prenatal) detection and surveillance of intra-amniotic infections. In the current study we questioned whether an intra-amniotic infection could be accurately diagnosed by a specific volatile organic compound (VOC) profile in exhaled breath of pregnant sheep. For this purpose pregnant Texel ewes were inoculated intra-amniotically with Ureaplasma parvum and serial collections of exhaled breath were performed for 6 days. Ureaplasma parvum infection induced a distinct VOC-signature in expired breath of pregnant sheep that was significantly different between day 0 and 1 vs. day 5 and 6. Based on a profile of only 15 discriminatory volatiles, animals could correctly be classified as either infected (day 5 and 6) or not (day 0 and 1) with a sensitivity of 83% and a specificity of 71% and an area under the curve of 0.93. Chemical identification of these distinct VOCs revealed the presence of a lipid peroxidation marker nonanal and various hydrocarbons including n-undecane and n-dodecane. These data indicate that intra-amniotic infections can be detected by VOC analyses of exhaled breath and might provide insight into temporal dynamics of intra-amniotic infection and its underlying pathways. In particular, several of these volatiles are associated with enhanced oxidative stress and undecane and dodecane have been reported as predictive biomarker of spontaneous preterm birth in humans. Applying VOC analysis for the early detection of intra-amniotic infections will lead to appropriate surveillance of these high-risk pregnancies, thereby facilitating appropriate clinical course of action including early treatment of preventative measures for pre-maturity-associated morbidities. |
| Databáze: | OpenAIRE |
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