Rapid quality control of 99Tcm-sestamibi
| Autor: | Sylvain Houle, Jeannie Polihronis, So M, Raymond M. Reilly |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Quality Control
Technetium Tc 99m Sestamibi Measurement method Chromatography Time Factors Chemistry business.industry General Medicine Reversed-phase chromatography Paramagnetic Contrast Agent respiratory system respiratory tract diseases Thin layer chromatographic lipids (amino acids peptides and proteins) Radiology Nuclear Medicine and imaging Nuclear medicine business Technetium-99m circulatory and respiratory physiology |
| Zdroj: | Nuclear medicine communications. 13(9) |
| ISSN: | 0143-3636 |
| Popis: | The thin layer chromatographic (TLC) method for determining the radiochemical purity of 99Tcm-sestamibi suggested by the manufacturer of the kit is slow and inconvenient. In this study a more rapid reversed phase chromatography (Sep-Pak) technique is compared with the TLC method for measurement of the radiochemical purity of 99Tcm-sestamibi. The levels of free 99Tcm-pertechnetate in 99Tcm-sestamibi were accurately determined using the Sep-Pak technique. However, due to binding of variable amounts of reduced-hydrolysed unbound 99Tcm to the chromatography cartridge, the Sep-Pak method overestimated the radiochemical purity of the radiopharmaceutical by approximately 3%. In practice, this overestimation is not important and the Sep-Pak technique can be used as a rapid method of determining radiochemical purity of 99Tcm-sestamibi. |
| Databáze: | OpenAIRE |
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