A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films
| Autor: | Alexander E Hampe, Zidong Li, Erkin Seker, Sunjay Sethi, Pamela J. Lein |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
microfluidic flow-cell Nanostructure Morphology (linguistics) Materials science nanostructure General Chemical Engineering Microfluidics nanoporous gold Bioengineering 02 engineering and technology lcsh:Chemistry Focal adhesion 03 medical and health sciences astrocyte Nanotechnology General Materials Science Thin film focal adhesion Nanoporous Communication Adhesion Materials Engineering 021001 nanoscience & nanotechnology adhesion strength 030104 developmental biology lcsh:QD1-999 Electrode Biophysics cell-material interaction 0210 nano-technology |
| Zdroj: | Nanomaterials (Basel, Switzerland), vol 8, iss 7 Nanomaterials Hampe, Alexander E; Li, Zidong; Sethi, Sunjay; Lein, Pamela J; & Seker, Erkin. (2018). A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films.. Nanomaterials (Basel, Switzerland), 8(7), 452-452. doi: 10.3390/nano8070452. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/96p1b5n1 Nanomaterials, Vol 8, Iss 7, p 452 (2018) |
| DOI: | 10.3390/nano8070452. |
| Popis: | Nanoporous gold (np-Au) electrode coatings have shown improved neural electrophysiological recording fidelity in vitro, in part due to reduced surface coverage by astrocytes. This reduction in astrocytic spreading has been attributed to the influence of electrode nanostructure on focal adhesion (FA) formation. This study describes the development and use of a microfluidic flow cell for imposing controllable hydrodynamic shear on astrocytes cultured on gold surfaces of different morphologies, in order to study the influence of nanostructure on astrocyte adhesion strength as a function of np-Au electrode morphology. Astrocyte detachment (a surrogate for adhesion strength) monotonically increased as feature size was reduced from planar surfaces to np-Au, demonstrating that adhesion strength is dependent on nanostructure. Putative mechanisms responsible for this nanostructure-driven detachment phenomenon are also discussed. |
| Databáze: | OpenAIRE |
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