Two-Step Enzymatic Conversion of Rebaudioside A into a Mono-α-1,4-Glucosylated Rebaudioside A Derivative
| Autor: | Peng Peng, Du Guoying, Li Xu, Yajing Sun, Guofeng Gu, Wang Wenzheng, Min Xiao |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Magnetic Resonance Spectroscopy Chromatography biology Chemistry 010401 analytical chemistry Two step General Chemistry Cyclodextrin glycosyltransferase 01 natural sciences 0104 chemical sciences Catalysis Hydrolysis Enzyme Yield (chemistry) biology.protein Stevia Glycosides Amylase Diterpenes Kaurane General Agricultural and Biological Sciences Rebaudioside A 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 69:2522-2530 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | A new two-step enzymatic conversion process for the production of a novel mono-α-1,4-glucosylated rebaudioside A derivative (RA1G) was established via transglycosylation followed by hydrolyzation. In the transglycosylation reaction catalyzed by cyclodextrin glycosyltransferase, rebaudioside A (RA) was converted into glucosylated RA derivatives (RAGs), and the maximum conversion of 87.8% was obtained with the optimal conditions of 2 U/mL enzyme, 82.5 mg/mL β-cyclodextrin, and 82.5 mg/mL RA at 60 °C for 5 h. The obtained RAG solution was then directly used in hydrolyzation. Four amylases were screened for shortening the newly synthesized α-glucans of RAGs. A glucoamylase was found to produce RA1G as the single glucosylated product, and the maximum yield of 53.3% was achieved with the optimal conditions of adding 1.5 U/mL glucoamylase into RAG solution at 60 °C for 3 h. RA1G was identified as 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl) oxy] ent-kaur-16-en-19-oic acid-[(4-O-α-D-glucopyranosyl-β-D-glucopyranosyl) ester] by MS and NMR analysis and showed an improved sensory quality compared to RA. |
| Databáze: | OpenAIRE |
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