Characterization of homogeneously staining regions in a small cell lung cancer cell line, using in situ hybridization with anMYCN probe
| Autor: | Suzanne Hasthorpe, Erin Dietzsch, O. Margaret Garson, Robyn Lukeis, Vickie Vrazas |
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| Rok vydání: | 1994 |
| Předmět: |
Cancer Research
Lung Neoplasms Marker chromosome Genes myc In situ hybridization Biology Tumor Cells Cultured Genetics medicine Humans Carcinoma Small Cell Gene In Situ Hybridization Aged Chromosome Cancer Karyotype medicine.disease Molecular biology Chromosome Banding Staining Blotting Southern Cell culture Karyotyping Female Oligonucleotide Probes |
| Zdroj: | Genes, Chromosomes and Cancer. 10:213-216 |
| ISSN: | 1098-2264 1045-2257 |
| Popis: | The cell line CIPL38 was derived from the pleural effusion of a patient with small cell lung cancer. The karyotype was hyperdiploid and complex with a variable number of marker chromosomes. Two of the markers had large homogeneously staining regions (hsr), which were shown to consist of amplified MYCN by in situ hybridization. One hsr bearing a marker chromosome could not be identified with G-banding, but the other was situated on a der(14). This was elucidated further with FISH analysis, which enabled the identification of sequences of chromosome I involved in a complex rearrangement with chromosome 14 and the hsr. Genes Chromosom Cancer 10:213–216 (1994). © 1994 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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