Probiotic characteristics of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum

Autor: Ayhan Temiz, Mustafa Ay, Ufuk Bagci, Sine Özmen Toğay
Přispěvatelé: Bursa Uludağ Üniversitesi/Ziraat Fakültesi/Gıda Mühendisliği/Gıda Bilimleri Bölümü., Togay, Sine Ozmen, AAC-6337-2021
Rok vydání: 2018
Předmět:
Antibiotic resistance
Physiology
Enterococcus faecium
Gel-electrophoresis
Cholesterol assimilation
Antimicrobial resistance
medicine.disease_cause
Bacterial Adhesion
law.invention
In-vitro
Bacterium adherence
Probiotic
Bacteriocins
law
Antiinfective agent
Gastric Juice
Biotechnology & applied microbiology
biology
food and beverages
Kanamycin
Microbial sensitivity test
General Medicine
Breast-milk
Anti-Bacterial Agents
Cholesterol
Oncorhynchus-mykiss
Human
medicine.drug
Breast milk
Bacteriocin
Tetracycline
Stomach juice
Bile acid
Microbial Sensitivity Tests
Probiotic agent
Lactic-acid bacteria
Microbiology
Bile Acids and Salts
Listeria monocytogenes
Caco-2 cell line
Antibiosis
Drug Resistance
Bacterial
Genetics
medicine
Humans
Milk
Human
Probiotic Agent
Lactobacillales
Colostrum
Probiotics
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Rainbow-trout
Drug effect
Antibiotic-resistant enterococci
Metabolism
Isolation and purification
Enterococcus
Virulence genes
Caco-2 Cells
Zdroj: Folia microbiologica. 64(6)
ISSN: 1874-9356
Popis: As potential probiotic traits of human milk-isolated bacteria have increasingly been recognized, this study aimed to evaluate the probiotic properties of bacteriocin-producing Enterococcus faecium strains isolated from human milk and colostrum. Among 118 human milk- and colostrum-isolated lactic cocci, only 29 were identified as Enterococcus. Of these, only four Enterococcus faecium isolates exhibited bacteriocigenic activity against several pathogenic Gram-positive bacteria, including Listeria monocytogenes. These isolates exhibited high acid (up to pH 3.0) and bile tolerance (0.5% oxgall) in simulated gastrointestinal conditions, demonstrating their ability to survive through the upper gastrointestinal tract. All of the E. faecium strains were shown to be sensitive to most of the antibiotics including vancomycin, tetracycline, rifampicin, and erythromycin, while they were resistant to kanamycin and chloramphenicol. None of the strains showed any virulence (gelE, agg2, clyA, clyB, clyM) and antibiotic resistance genes (vanA, vanB, ermB, tetM, and aac(6')-le-aph(2 '')-la). In addition, all the strains were able to assimilate cholesterol, ranging between 25.2-64.1% and they exhibited variable adherence (19-36%) to Caco-2 cells. Based on the overall results of this in vitro study, four of the E. faecium strains isolated from human milk and colostrum can be considered as promising probiotic candidates; however, further in vivo evaluations are required. Hacettepe Üniversitesi 4753
Databáze: OpenAIRE
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