Psychosocial stress inhibits additional stress-induced hyperexpression of NO synthases and IL-1β in brain structures
| Autor: | Jan Bugajski, Anna Gądek-Michalska, Paulina Rachwalska, Joanna Tadeusz |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Interleukin-1beta Nitric Oxide Synthase Type II Hippocampus Nitric Oxide Synthase Type I Nitric oxide Random Allocation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot Internal medicine medicine Animals Chronic stress Rats Wistar Prefrontal cortex Pharmacology biology medicine.diagnostic_test Brain General Medicine Housing Animal Rats Nitric oxide synthase 030104 developmental biology Endocrinology Gene Expression Regulation chemistry Hypothalamus Psychosocial stress biology.protein Stress Psychological 030217 neurology & neurosurgery |
| Zdroj: | Pharmacological Reports. 68:1178-1196 |
| ISSN: | 1734-1140 |
| DOI: | 10.1016/j.pharep.2016.09.003 |
| Popis: | Background The aim of this study was to compare the expression of interleukin-1β (IL-1β), neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) in the prefrontal cortex (PFC), hippocampus (HIP) and hypothalamus (HT) during chronic crowding (CS) (psychosocial) and restraint (RS) (physico-psychological) stress. Adaptational changes of these stress mediators to a subsequent acute RS, in two models of chronic stress were investigated. Methods Rats were crowded (24 in one cage) or restrained in metal tubes for 10 min twice a day for 3, 7, and 14 consecutive days and decapitated. For determination of adaptational changes the chronically crowded and restrained rats 24 h after the last stress session were subjected to a single 10 min RS. The IL-1β, nNOS and iNOS protein levels in brain structures samples were analyzed by Western blot procedure. Results Chronic CS for 3 days did not markedly change the subsequent acute stress induced expression of nNOS, iNOS and IL-1β protein level in PFC and iNOS protein level in HT. CS markedly decreased the expression of nNOS, iNOS and IL-1β in HIP. By contrast, parallel chronic RS, significantly increased the subsequent acute stress-induced expression of iNOS and IL-1β in PFC and considerably increased iNOS level in HT. Conclusion Chronic psychosocial stress, may protect against possible harmful action of hyperproduction of iNOS and iNOS derived nitric oxide (NO) mainly in PFC and HIP. By contrast, chronic physico-psychosocial stress may strongly potentiate additional stress-induced harmful effects of NOS and IL-1β hyperproduction. |
| Databáze: | OpenAIRE |
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