Differences in the Biological Activities of Transforming Growth Factor-β and Platelet-Derived Growth Factorin vivo
| Autor: | Steven J. Sawamura, Paula D. Avis, Carolyn O. Gerhardt, John M. McPherson, Bruce M. Pratt, Yasushi Ogawa, George A. Ksander, Mark J. Murray, Jill M. Ziman |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Platelet-derived growth factor Guinea Pigs Clinical Biochemistry law.invention Glycosaminoglycan chemistry.chemical_compound Endocrinology Transforming Growth Factor beta In vivo law Animals Connective Tissue Cells Glycosaminoglycans Platelet-Derived Growth Factor biology Chemistry Cell Biology Transforming growth factor beta Molecular biology Recombinant Proteins Kinetics Biochemistry Connective Tissue biology.protein Recombinant DNA Wound healing Platelet-derived growth factor receptor DNA |
| Zdroj: | Growth Factors. 5:57-68 |
| ISSN: | 1029-2292 0897-7194 |
| DOI: | 10.3109/08977199109000271 |
| Popis: | Transforming growth factor-beta 1 (TGF-beta 1 and recombinant platelet-derived growth factor-BB (rPDGF-BB) promoted an extensive, dose-dependent development of fibrous connective tissue when continuously delivered for 8 days by mini-osmotic pumps implanted subcutaneously in adult guinea pigs. Biochemical analyses demonstrated that TGF-beta 1 and rPDGF-BB stimulated dose-dependent increases in the dry weight, and protein, DNA, collagen, and glycosaminoglycan (GAG) contents of the fibrous connective tissue capsule that enveloped the pumps. The GAG/DNA mass ratio was markedly elevated by TGF-beta 1, but the collagen/DNA, protein/DNA, and collagen/protein ratios were not significantly increased. In contrast, rPDGF-BB generally decreased these mass ratios. Histological analyses suggested that this was due to the fact that rPDGF-BB induced a very cellular response with a marked influx of neutrophils and fibroblasts. TGF-beta 1 induced significantly less cellular response, which consisted primarily fibroblasts and macrophages. These results indicated that rPDGF-BB and TGF-beta 1 induced connective tissue deposition in vivo in a dose-dependent fashion, although the cellular nature of the responses as well as the structural composition of the extracellular matrices were clearly distinguishable between the two growth factors. |
| Databáze: | OpenAIRE |
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