Administration of Ginkgo biloba Extract (EGb761) Alone and in Combination with FK506 Promotes Liver Regeneration in a Rat Model of Partial Hepatectomy
| Autor: | Sabahattin Muhtaroglu, Abdulkadir Bedirli, Nahide Ekici Günay |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Mitotic index medicine.medical_treatment FK506 lcsh:Medicine liver medicine.disease_cause Tacrolimus EGb761 03 medical and health sciences chemistry.chemical_compound hepatectomy 0302 clinical medicine Internal medicine thymidine kinase medicine Animals oxidative stress Rats Wistar Free-radical theory of aging mitosis EGb761 FK506 hepatectomy liver oxidative stress regeneration thymidine kinase mitosis Dose-Response Relationship Drug biology Plant Extracts Chemistry Ginkgo biloba lcsh:R General Medicine biology.organism_classification Malondialdehyde Liver regeneration Liver Regeneration Rats 030104 developmental biology medicine.anatomical_structure Endocrinology regeneration 030220 oncology & carcinogenesis Hepatocyte Original Article Drug Therapy Combination Hepatectomy Oxidative stress |
| Zdroj: | Balkan Medical Journal, Vol 35, Iss 2, Pp 174-180 (2018) Balkan Medical Journal Volume: 35, Issue: 2 174-180 |
| ISSN: | 2146-3131 2146-3123 |
| Popis: | Background: Free radical damage is known to occur during liver regeneration. The Ginkgo biloba extract EGb761 has antioxidant properties due to its ability to scavenge free radicals. FK506 has been widely used as an immunosuppressant that stimulates hepatocyte proliferation following partial hepatectomy.Aims: To explore whether EGb761 enhances liver regeneration after hepatectomy in rats, we investigated the effects of EGb761 alone and in combination with FK506 on the liver regenerative process.Study Design: Animal experimentation.Methods: A total of 75 Wistar albino rats weighing 340.08±11.66 g were randomly divided into five experimental groups: sham, control, FK506, EGb761, and FK506 + EGb761. According to the study groups, rats were administered FK506 at a dose of 0.1 mg/kg/day and EGb761 at 25 mg/kg/day three times via the intraperitoneal route. Then, two-thirds hepatectomy was performed according to the Higgins and Anderson technique in all the rats. At postoperative 48 h, 53 surviving rats were sacrificed. Serum and plasma samples were collected for analyzing thymidine kinase and oxidative stress marker levels. The regenerated liver was entirely resected, weighed, and sectioned. The mitotic index was assessed using hematoxylin-eosin staining. The extent of liver regeneration was calculated using the Child’s formula. The data were statistically analyzed using ANOVA, with a significance level of 5% (p |
| Databáze: | OpenAIRE |
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