Acute and sub-chronic toxicity study of the extract and powder of Operculina macrocarpa (L.) Urb. in mice
| Autor: | José Galberto Martins da Costa, MarcÃlio Fontes Cezar, FabrÃcio Kleber de Lucena Carvalho, Onaldo Guedes Rodrigues, Sérgio Santos de Azevedo, Ana Célia Rodrigues Athayde, Carpejane Ferreira da Silva |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
010405 organic chemistry
business.industry Pharmacology Body weight 01 natural sciences Applied Microbiology and Biotechnology Median lethal dose 0104 chemical sciences Subchronic toxicity Operculina macrocarpa 010404 medicinal & biomolecular chemistry Phytochemical Distilled water Oral administration Mice Operculina macrocarpa hematological parameters toxicity Toxicity Genetics Medicine business Agronomy and Crop Science Molecular Biology Biotechnology |
| Zdroj: | African Journal of Biotechnology; Vol 15, No 51 (2016); 2776-2783 |
| ISSN: | 1684-5315 |
| DOI: | 10.5897/ajb2016.15669 |
| Popis: | The aim of the study was to evaluate the experimental acute and sub-chronic toxicities of Operculina macrocarpa with plant extract and powder, respectively, in male and female mice. Phytochemical prospection was performed with extract and administration in single doses by intraperitoneal route to six groups and control at 1230, 970, 700, 350, 120 and 30 mg/kg doses and distilled water (0.1 ml/10 g), respectively. Sub-chronic doses of 1230, 700, 30 mg/kg/day and satellite (1230 mg/kg/day) were administered orally in feed. Major endpoints included alterations in the central and autonomic nervous system, water and food intake, body weight, hematological and biochemical parameters. Phytochemical screening identified compounds: Alkaloids, flavonoids, xanthones, leucoanthocyanidins and tannins condensate. In the acute study, mortality was observed with toxicity signs to the central nervous system (CNS) at LD 50 of 270 mg/kg. There were no significant changes in water and food intake, body weight, haematological and biochemical parameters, and histopathological examination in the subchronic study (p value). Results indicate that the oral administration of O. macrocarpa powder in feed is less toxic and relatively safer. Key words : Mice, Operculina macrocarpa, hematological parameters, toxicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|