Karyological analysis of an interspecific hybrid between the dioeciousSilene latifoliaand the hermaphroditicSilene viscosa
| Autor: | Michaela Marková, Boris Vyskot, Bohuslav Janousek, Jitka Zluvova, Martina Lengerová |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
X Chromosome
Disorders of Sex Development medicine.disease_cause Genome Chromosomes Plant Meiosis Chromosome Segregation Pollen Botany Genetics medicine Silene latifolia Silene Molecular Biology Crosses Genetic In Situ Hybridization Fluorescence Phylogeny X chromosome biology medicine.diagnostic_test Chimera General Medicine Interspecific competition biology.organism_classification Karyotyping Genome Plant Biotechnology Fluorescence in situ hybridization |
| Zdroj: | Genome. 49:373-379 |
| ISSN: | 1480-3321 0831-2796 |
| Popis: | The genus Silene is a good model for studying evolution of the sex chromosomes, since it includes species that are hermaphroditic and dioecious, while maintain a basic chromosome number of 2n = 24. For some combinations of Silene species it is possible to construct interspecific hybrids. Here, we present a detailed karyological analysis of a hybrid between the dioecious Silene latifolia as the maternal plant and a related species, hermaphroditic Silene viscosa, used as a pollen partner. Using genomic probes (the genomic in situ hybridization (GISH) technique), we were able to clearly discriminate parental genomes and to show that they are largely separated in distinct nuclear domains. Molecular GISH and fluorescence in situ hybridization (FISH) markers document that the hybrid genome of somatic cells was strictly additive and stable, and that it had 12 chromosomes originating from each parent, including the only X chromosome of S. latifolia. Meiotic analysis revealed that, although related, respective parental chromosomes did not pair or paired only partially, which resulted in frequent chromosome abnormalities such as bridges and irregular non-disjunctions. GISH and FISH markers clearly document that the larger genome of S. latifolia and its largest chromosome component, the X chromosome, were mostly employed in chromosome lagging and misdivision.Key words: sex chromosome, Silene, interspecific hybrid, meiotic pairing, misdivision. |
| Databáze: | OpenAIRE |
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