Electrophoretic separation of the synovial fluid proteins in patients with temporomandibular joint disorders
| Autor: | Natsuki Segami, Jun Sato, Yoshino Yoshitake, Naoki Tsuruoka, Susumu Kobayashi, Kazuma Fujimura, Keiseki Kaneyama |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Adolescent Joint Dislocations Osteoarthritis Asymptomatic Statistics Nonparametric Internal medicine Synovial Fluid Arthropathy medicine Humans Synovial fluid General Dentistry Aged Gel electrophoresis Chemistry Proteins Middle Aged Temporomandibular Joint Disorders Joint effusion medicine.disease Pathophysiology Temporomandibular joint Molecular Weight stomatognathic diseases Endocrinology medicine.anatomical_structure Otorhinolaryngology Case-Control Studies Electrophoresis Polyacrylamide Gel Female Surgery Oral Surgery medicine.symptom |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 101:463-468 |
| ISSN: | 1079-2104 |
| Popis: | The present study was performed to characterize the patterns of protein expression in the synovial fluid (SF) of patients with temporomandibular joint disorders (TMD) by electrophoretic fractionation.Samples of the SF of 26 consecutive patients consisting of 16 with closed locking (CL group) and 10 with osteoarthritis (OA group), as well as 7 asymptomatic control subjects (AS group), were analyzed in the present study. SF samples were obtained from the upper compartment of the temporomandibular joint (TMJ) and equal quantities of SF protein were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).The mean total protein concentrations in the SF from both of the TMD groups were higher than that in the AS group (1353 microg/mL in the CL group and 2485 microg/mL in the OA group vs 615 microg/mL in the AS group; P.01). Moreover, the mean total SF protein concentration was higher in the OA group than in the CL group (P.01). There was a correlation between the total protein concentration in the SF from both patient groups and the degree of expanded joint effusion (P = .003, r = 0.685). Approximately 22 different protein bands with molecular weights ranging from 14 to 700 kd were clearly discernible on electrophoresis. The relative amounts of specific proteins in the SF of the TMD group were also different from those in the AS group (P.05). The major difference in total protein concentration appeared to be due to the increased abundance of relatively high molecular weight proteins (140 kd) in the TMD patients as compared to the AS group.The SF of patients with TMD showed significant quantitative differences in total protein abundance as compared to healthy subjects. Moreover, this protein abundance was correlated strongly with the degree of expanded joint effusion. The major difference in total protein concentration appeared to be due to the increased abundance of relatively high molecular weight polypeptides in the TMD patients as compared to the healthy control subjects. These observations of changes in the pattern of protein expression may help in understanding the etiological factors involved in the pathophysiology of TMD. |
| Databáze: | OpenAIRE |
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