Incorporation of [35S]sulphate into brain constituents during development and the effects of thyroid hormone on myelination
| Autor: | B.W.L. Brooksbank, D.A. Wilson, Robert Balázs, Anthony L. Johnson, A.J. Patel |
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| Rok vydání: | 1971 |
| Předmět: |
Male
Thyroid Hormones medicine.medical_specialty Glycosaminoglycan Myelin Cerebrosides Cerebellum Internal medicine Sulfur Isotopes medicine Animals Molecular Biology Myelin Sheath Triiodothyronine Sulfates Chemistry General Neuroscience Thyroid Age Factors Brain Organ Size Lipid Metabolism Rat brain Cerebroside Rats medicine.anatomical_structure Endocrinology Age distribution Neurology (clinical) Developmental Biology Hormone |
| Zdroj: | Brain Research. 30:273-293 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(71)90079-5 |
| Popis: | Summary The effect of triiodothyronine (T 3 ) on cerebroside deposition and on the incorporation of [ 35 S]sulphate into lipids was studied in the rat brain between 9 and 26 days after birth. Cerebroside depositions was accelerated to a small extent and only before 17 days of age. The incorporation of 35 S into brain lipids was maximal at 17 days, but the peak was less pronounced in the T 3 -treated than in the control animals. At the age the specific radioactivity of the separated [ 35 S]sulphatides was reduced by treatment with T 3 , but the reduction was no longer apparent when the data were expressed in terms of the concentration of acid-soluble 35 S. There was also an anomaly even in the control between the rates of cerebroside deposition and of the incorporation of [ 35 S]sulphate into lipids during the period from 17 to 26 days. It was concluded that the content of [ 35 S]sulphatides is not a valid measure of myelin deposition during development, unless the kinetics of incorporation of 35 S into the brain are also taken into account. The investigation also included a study of the age distribution of 35 S in the fractions containing mucopolysaccharides and acid-soluble constituents in the brains of both the control and T 3 -treated animals. The maximum content of 35 S occurred at 17 days in the fraction containing mucopolysaccharides but at 13 days in the acid-soluble fraction. The result showed that factors determining the availability of precursor sulphate must be considered in assessing relative rates of 35 S incorporation. |
| Databáze: | OpenAIRE |
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