Influence of Supplementation of Vegetable Oil Blends on Omega-3 Fatty Acid Production in Mortierella alpina CFR-GV15
| Autor: | Pamidighantam Prabhakara Rao, G. Venkateswaran, Ganesan Vadivelan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
food.ingredient Article Subject lcsh:Medicine Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound food Linseed oil Botany Food science Omega 3 fatty acid chemistry.chemical_classification General Immunology and Microbiology lcsh:R Fatty acid food and beverages General Medicine Eicosapentaenoic acid 030104 developmental biology Vegetable oil chemistry Docosahexaenoic acid Arachidonic acid Polyunsaturated fatty acid |
| Zdroj: | BioMed Research International, Vol 2017 (2017) |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2017/1432970 |
| Popis: | Objectives of this study were designed for improved production of mycelial omega-3 fatty acids with particular reference to EPA and DHA from the oleaginous fungusMortierella alpinaCFR-GV15 under submerged low temperatures fermentation supplemented with linseed oil and garden cress oil as an additional energy source. The fungus was grown at 20°C temperature for four days initially followed by 12°C temperature for next five days. The basal medium contained starch, yeast extract, and a blend of linseed oil (LSO) and garden cress oil (GCO) in the ratio 1 : 1. Results of the study revealed that, after nine days of total incubation period, the enhancement of biomass was up to 16.7 g/L dry weight with a total lipid content of 55.4% (v/w). Enrichment of omega-3 fatty acids indicated a significant increase in fatty acid bioconversion (ALA32.2±0.42%, EPA7.9±0.1%, and DHA4.09±0.2%) by 2.5-fold. The two-stage temperature cultivation alters the fatty acid profile due to activation of the desaturase enzyme in the cellular levels due to which arachidonic acid (AA) content reduced significantly. It can be concluded thatMortierella alpinaCFR-GV15 is a fungal culture suitable for commercial production of PUFAs with enriched EPA and DHA. |
| Databáze: | OpenAIRE |
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