DNA methylation screening after roux-en Y gastric bypass reveals the epigenetic signature stems from genes related to the surgery per se
| Autor: | Angel Diaz-Lagares, Carolina Ferreira Nicoletti, F. F. Casanueva, M. A. S. Pinhel, Carla Barbosa Nonino, B. A. P. de Oliveira, Ana B. Crujeiras, A. Jácome, Vitor Caressato Pinhanelli |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty lcsh:Internal medicine Weight loss Time Factors lcsh:QH426-470 Angiogenesis Gastric Bypass Inflammation Epigenesis Genetic 03 medical and health sciences 0302 clinical medicine Genetics medicine Humans Epigenetics Obesity lcsh:RC31-1245 Genetics (clinical) Bariatric surgery DNA methylation business.industry Body Weight Wnt signaling pathway nutritional and metabolic diseases Methylation Surgery lcsh:Genetics 030104 developmental biology Phenotype CpG site 030220 oncology & carcinogenesis CpG Islands Female medicine.symptom business Research Article Pathway |
| Zdroj: | BMC Medical Genomics BMC Medical Genomics, Vol 12, Iss 1, Pp 1-12 (2019) |
| ISSN: | 1755-8794 |
| Popis: | Background/objectives Obesity has been associated with gene methylation regulation. Recent studies have shown that epigenetic signature plays a role in metabolic homeostasis after Roux-en Y gastric bypass (RYGB). To conduct a genome-wide epigenetic analysis in peripheral blood to investigate whether epigenetic changes following RYGB stem from weight loss or the surgical procedure per se. Subjects/methods By means of the Infinium Human Methylation 450 BeadChip array, global methylation was analyzed in blood of 24 severely obese women before and 6 months after RYGB and in 24 normal-weight women (controls). Results In blood cells, nine DMCpG sites showed low methylation levels before surgery, methylation levels increased after RYGB and neared the levels measured in the controls. Additionally, 44 CpG sites associated with the Wnt and p53 signaling pathways were always differently methylated in the severely obese patients as compared to the controls and were not influenced by RYGB. Finally, 1638 CpG sites related to inflammation, angiogenesis, and apoptosis presented distinct methylation in the post-surgery patients as compared to the controls. Conclusion Bariatric surgery per se acts on CpGs related to inflammation, angiogenesis, and endothelin-signaling. However, the gene cluster associated with obesity remains unchanged, suggesting that weight loss 6 months after RYGB surgery cannot promote this effect. Graphical abstract Electronic supplementary material The online version of this article (10.1186/s12920-019-0522-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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