NMR studies of intracellular water at 300 MHz:T2-specific relaxation mechanisms in synchronized or EGF-stimulated cells
| Autor: | Scott F. Deamond, D. E. Callahan, Paul O. P. Ts'o, Lou-Sing Kan, D. C. Creasey, Tina L. Trapane, Sarah A. Bruce |
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| Rok vydání: | 1991 |
| Předmět: |
Intracellular Fluid
Cell physiology Magnetic Resonance Spectroscopy Epidermal Growth Factor Chemistry Hamster In Vitro Techniques Stimulation Chemical In vitro Cell Line Spin–spin relaxation Nuclear magnetic resonance Epidermal growth factor Cell culture Cricetinae Biophysics Animals Radiology Nuclear Medicine and imaging Cytoskeleton Intracellular Cell Line Transformed |
| Zdroj: | Magnetic Resonance in Medicine. 22:68-80 |
| ISSN: | 1522-2594 0740-3194 |
| DOI: | 10.1002/mrm.1910220108 |
| Popis: | Responses specific to the spin-spin relaxation time (T2) have been observed in two time-dependent studies of the intracellular water in normal and transformed Syrian hamster fetal fibroblasts. At 300-MHz (7.0 T), the spin-lattice relaxation time (T1) was insensitive to several aspects of cellular physiology that produced changes in the T2 and the apparent self-diffusion coefficient (Dapp) of intracellular water. In normal cells stimulated with epidermal growth factor (EGF), T1 was insensitive to time-dependent changes detected by T2 and Dapp. In synchronized tumor cells, T1 was insensitive to cell-cycle-dependent changes detected by T2. The strongly coupled behavior of T2 and Dapp that was observed as a function of time in EGF-stimulated cells indicates that the diffusion of intracellular water through inhomogeneous local magnetic field gradients produced effects observable in T2. Conformational changes in large intracellular macromolecular assemblies such as chromatin or the cytoskeleton may alter the magnitude and inhomogeneity of local field gradients, producing responses in T2 and Dapp only. © 1991 Academic Press, Inc. |
| Databáze: | OpenAIRE |
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