Intracellular localization of influenza C virus NS2 protein (NEP) in infected cells and its incorporation into virions
| Autor: | Emi Takashita, Seiji Hongo, Yoshihiko Kohno, Kanetsu Sugawara, Yasushi Muraki, Yoko Matsuzaki |
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| Rok vydání: | 2009 |
| Předmět: |
Cytoplasm
Influenzavirus C viruses Orthomyxoviridae Active Transport Cell Nucleus Chick Embryo Viral Nonstructural Proteins Virus Replication Virus Cell Line Virology Influenza Human Animals Humans Nuclear export signal Cell Nucleus biology Cell Membrane Virion virus diseases General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology Nucleoprotein Ribonucleoproteins Viral replication Influenza C Virus |
| Zdroj: | Archives of Virology. 154:235-243 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | RNA segment 7 of influenza C virus encodes two non-structural (NS) proteins, NS1 and NS2. The influenza C virus NS2 protein has been proposed to possess nuclear export activity like that of influenza A and B virus NS2 proteins (NEP). In the present study, we investigated the kinetics and localization of the NS2 protein in influenza C virus-infected cells, and analysed whether NS2 is present in virions. Immunofluorescent staining analysis of the infected cells indicated that NS2 was localized in the nucleus immediately after synthesis and predominantly in the cytoplasm in the later stages of infection. Confocal microscopy revealed that a part of the NS2 protein was colocalized with nucleoprotein NP/vRNP in the cytoplasm and on the cell membrane in the late stages of infection. The NS2 protein was detected in influenza C virions purified by gradient centrifugations and/or affinity chromatography. Trypsin treatment demonstrated that the NS2 protein was present inside the viral envelope. Furthermore, glycerol gradient analysis of detergent-solubilized virions revealed that the NS2 protein cosedimented with vRNPs. These data suggest that the influenza C virus NS2 protein is incorporated into virions, where it associates with vRNP. |
| Databáze: | OpenAIRE |
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