The mouse keratin 6 isoforms are differentially expressed in the hair follicle, footpad, tongue and activated epidermis
| Autor: | Masashi Ogo, Donnie S. Bundman, Dennis R. Roop, Joseph A. Rothnagel, Toshihiko Seki, Jackie R. Bickenbach, Mary A. Longley, Sonja M. Wojcik |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Gene isoform
Cancer Research Transcription Genetic Genetic Linkage Transgene Cellular differentiation Blotting Western Fluorescent Antibody Technique Mice Transgenic Biology Mice Tongue Keratin medicine Animals Protein Isoforms RNA Messenger Molecular Biology chemistry.chemical_classification Mice Inbred BALB C Epidermis (botany) Reverse Transcriptase Polymerase Chain Reaction Cell Biology Hair follicle Molecular biology Hindlimb medicine.anatomical_structure Gene Expression Regulation chemistry Regulatory sequence Keratins Epidermis Keratinocyte Hair Follicle Developmental Biology |
| Zdroj: | Differentiation. 65:119-130 |
| ISSN: | 0301-4681 |
| DOI: | 10.1046/j.1432-0436.1999.6520119.x |
| Popis: | Keratin 6 (K6) is expressed constitutively in a variety of internal stratified epithelia as well as in palmoplantar epidermis and in specialized cells of the hair follicle. K6 expression can also be induced by hyperproliferative conditions as in wound healing or by conditions that perturb normal keratinocyte function. The functional significance of the expression of K6 on keratinocyte biology under these disparate conditions is not known. Here we report on the characterization of two isoforms of mouse K6 that are encoded by separate genes. The two genes (denoted K6a and K6b) are linked, have the same orientation and are actively transcribed. Sequence analysis revealed, that although they encode almost identical products, they have distinctly different regulatory regions, suggesting that the two K6 genes would be differentially expressed. In an attempt to define the expression characteristics of the K6 isoforms, we produced transgenic mice with each gene after modifying the C-terminal sequences to enable detection of the transgenic proteins with specific antibodies. The constitutive expression of the K6a transgene paralleled that of the endogenous genes in all K6 expressing tissues, except in the tongue. The K6b transgene was also expressed in these tissues but, in contrast to K6a, was only expressed in suprabasal cells. Both K6 transgenes were also induced in the interfollicular epidermis in response to phorbol esters, with K6a induced in all layers of the treated epidermis, while K6b was expressed only in suprabasal cells. These studies suggest that the K6 isoforms have overlapping yet distinct expression profiles. |
| Databáze: | OpenAIRE |
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