Effects of Hg sublethal exposure in the brain of peacock blennies Salaria pavo: Molecular, physiological and histopathological analysis

Autor: Zohra Haouas, Ronny Blust, Patrick Kestemont, Ahmed Noureddine Helal, Benoît Chénais, Azza Naïja, Justine Marchand
Přispěvatelé: Bioressources: Integrative Biology and Valuation, Higher Institute of Biotechnology of Monastir (BIOLIVAL), Research Unit in Environmental and Evolutionary Biology (URBE-NARILIS), Université de Namur [Namur] (UNamur), Mer, molécules et santé EA 2160 (MMS), Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN), Laboratoire de genetique, Biodiversité et Environnement (UR 09/30), Institut Supérieur de Biotechnologie de Monastir (ISBM), Université de Monastir - University of Monastir (UM)-Université de Monastir - University of Monastir (UM), Universiteit Antwerpen [Antwerpen], Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN)-Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST)
Rok vydání: 2017
Předmět:
0301 basic medicine
Cerebellum
Salaria
Health
Toxicology and Mutagenesis
010501 environmental sciences
01 natural sciences
chemistry.chemical_compound
Peacock blenny
Multidrug resistance-associated protein 2
q-RT-PCR
Brain
General Medicine
Pollution
Acetylcholinesterase
Chemistry
medicine.anatomical_structure
Mercuric Chloride
language
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
Fish Proteins
medicine.medical_specialty
Environmental Engineering
Salaria pavo
Aché
HgCl
Histopathology
Biology
03 medical and health sciences
Hg concentration
Internal medicine
medicine
Environmental Chemistry
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
14. Life underwater
0105 earth and related environmental sciences
Messenger RNA
Public Health
Environmental and Occupational Health
mRNA expression
General Chemistry
Mercury
biology.organism_classification
language.human_language
Perciformes
030104 developmental biology
Endocrinology
chemistry
HgCl2
AChE
Zdroj: Chemosphere
Chemosphere, Elsevier, 2018, 193, pp.1094-1104. ⟨10.1016/j.chemosphere.2017.11.118⟩
ISSN: 1879-1298
0045-6535
DOI: 10.1016/j.chemosphere.2017.11.118⟩
Popis: Marine environments are affected by large amounts of toxicants among those mercury (Hg). The aim of this study was to assess potential neurotoxic effects of Hg in the peacock blenny Salaria pavo. A sublethal contamination to 66 jig HgCl2 L-1 over periods of 1, 4, 10 and 15 days was performed. Total Hg concentrations measured in the brain highlighted the detection of Hg at days 1 and 4 following the exposure but no concentration of the metal was further detected. Partial-length cDNA of genes coding ABC transporters (abcbl, abccl, abcc2, abcg2) and acetylcholinesterase (ache) were characterized. Results from mRNA expression levels displayed an up-regulation of abcbl mRNA while a down-regulation of abccl and abcc2 mRNA was observed. No change in abcg2 and ache mRNA expression was noted throughout the experiment. At each sampling time, Hg exposure did not affect the activity of the AChE enzyme. The histological analysis indicated that fish exhibited several damages in the optic tectum and the cerebellum and 3 reaction patterns were identified for each organ: circulatory disturbances, regressive and progressive changes. Molecular, physiological and histological biomarkers assessed in the present study highlighted that peacock blennies were able to detoxify Hg from the brain tissue by developing defense mechanisms. More globally, neurotoxic effects of a sublethal Hg exposure in the brain of peacock blennies and the adaptation capacity of this species were evaluated. (C) 2017 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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