Assessment of the pathway of apoptosis involving PAR-4, DAXX and ZIPK proteins in CLL patients and its relationship with the principal prognostic factors
| Autor: | Agata Surdacka, Agnieszka Bojarska-Junak, Anna Dmoszynska, Iwona Hus, Maria Luiza Kusz, Sylwia Chocholska, Malgorzata Sieklucka, Ewa Wąsik-Szczepanek, Jacek Roliński |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Histology Chronic lymphocytic leukemia PAWR BCL-2 Apoptosis PAR-4 Biology CD38 ZIPK Pathology and Forensic Medicine chemistry.chemical_compound Death-associated protein 6 Downregulation and upregulation Lactate dehydrogenase Biomarkers Tumor medicine Humans lcsh:QH573-671 B cell Adaptor Proteins Signal Transducing Aged Neoplasm Staging Aged 80 and over lcsh:Cytology Nuclear Proteins General Medicine Middle Aged MAP Kinase Kinase Kinases Prognosis medicine.disease Leukemia Lymphocytic Chronic B-Cell Cell biology medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 chemistry DAXX Cancer research chronic lymphocytic leukemia Female Apoptosis Regulatory Proteins Co-Repressor Proteins Molecular Chaperones |
| Zdroj: | Folia Histochemica et Cytobiologica, Vol 49, Iss 1, Pp 98-103 (2011) |
| ISSN: | 1897-5631 0239-8508 |
| DOI: | 10.5603/fhc.2011.0014 |
| Popis: | Par-4 (prostate apoptosis response-4) protein was originally found upregulated in prostate tumor cells undergoing apoptosis. Then it was further identified as a proapoptotic protein upregulated both in normal and leukemic lymphocytes. The aim of our study was to assess PAR-4 protein expression in the B cells of CLL patients and to examine its relationship with the expression of other proteins involved in the apoptosis process, such as DAXX, ZIPK and BCL-2. We found a positive relationship between PAR-4 and BCL-2 protein expression. Additionally, there was a positive correlation between PAR-4 and both DAXX and ZIPK protein expression. The results of our research were also analyzed in association with the principal CLL prognostic factors. There was a positive correlation between the expression of PAR-4 protein and the lactate dehydrogenase (LDH) serum concentration (p < 0.005). The expression of PAR-4 protein in B cells correlated positively with the percentage of CD38+ cells (p < 0.05), as well as with CD38+/ZAP-70+ cells (p < 0.05). Moreover, we found a close relationship between LPL protein expression or LPL/ADAM29 MFI ratio and PAR-4 protein expression. Our results confirm the significance of apoptosis deregulation in CLL, and suggest a possible relationship between PAR-4 expression and the clinical course of the disease. This however requires further investigation. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 98–103) |
| Databáze: | OpenAIRE |
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