| Autor: |
Vaibhav Kumar Pandya, S. Shiva Shankar, Babasaheb P. Sonwane, S. Rajesh, Rajeshwari Rathore, Sangaralingam Kumaran, Mahesh J. Kulkarni |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Biochimica et biophysica acta. General subjects. |
| ISSN: |
1872-8006 |
| Popis: |
Anserine and carnosine represent histidine-containing dipeptides that exert a pluripotent protective effect on human physiology. Anserine is known to protect against oxidative stress in diabetes and cardiovascular diseases. Human carnosinases (CN1 and CN2) are dipeptidases involved in the homeostasis of carnosine. In poikilothermic vertebrates, the anserinase enzyme is responsible for hydrolyzing anserine. However, there is no specific anserine hydrolyzing enzyme present in humans. In this study, we have systematically investigated the anserine hydrolyzing activity of human CN1 and CN2. A targeted multiple reaction monitoring (MRM) based approach was employed for studying the enzyme kinetics of CN1 and CN2 using carnosine and anserine as substrates. Surprisingly, both CN1 and CN2 can hydrolyze anserine effectively. The observed catalytic turnover rate (V |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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