Attraction of Adults of Cyclocephala lunulata and Cyclocephala barrerai (Coleoptera: Scarabaeoidea: Melolonthidae) towards Bacteria Volatiles Isolated from Their Genital Chambers
| Autor: | Angel Alonso Romero-López, Abraham Sanchez-Cruz, Norma Robledo, María Rosete-Enríquez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Microorganism Pharmaceutical Science Zoology Scarabaeoidea 01 natural sciences microbial natural products discovery Analytical Chemistry Electroantennography lcsh:QD241-441 03 medical and health sciences Cyclocephala lcsh:Organic chemistry Drug Discovery Physical and Theoretical Chemistry bioassays 030304 developmental biology 0303 health sciences biology volatile organic compounds from bacteria Organic Chemistry EAG biology.organism_classification Attraction 010602 entomology Olfactometer Chemistry (miscellaneous) Molecular Medicine Sex Attractants Bacteria attraction |
| Zdroj: | Molecules, Vol 25, Iss 4430, p 4430 (2020) Molecules Volume 25 Issue 19 |
| ISSN: | 1420-3049 |
| Popis: | In the study of the chemical communication of adults of the Melolonthidae family, bacteria have been observed in the epithelium of the genital chamber possibly, bacteria are involved in the production of sex attractants in their hosts. Therefore, it is important to identify the volatile organic compounds from bacteria (VOCsB) released by these microorganisms and study the biological activity stimulated by VOBCs in adults of Melolonthidae. In this study, bacteria were isolated from the genital chamber of Cyclocephala lunulata and Cyclocephala barrerai, from which VOCsB were extracted using static headspace solid-phase microextraction (SHS-SPME) and dynamic headspace Super Q solid-phase extraction (DHS-SPE) and analyzed using gas chromatography-mass spectrometry. The effect of VOCsB on the hosts and conspecifics was evaluated utilizing an olfactometer and electroantennography (EAG). Two species of Enterobacteria were isolated from the genital chamber of each female species, and VOCsB derived from sulfur-containing compounds, alcohols, esters, and fatty acids were identified. An attraction response was observed in olfactometry studies, and antennal responses to VOCsB were confirmed in EAG bioassays. With these results, new perspectives on the relationship between these beetles and their bacteria emerge, in addition to establishing a basis for management programs in the future. |
| Databáze: | OpenAIRE |
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