Retinoid X receptor beta (RXRB) expression in Sertoli cells controls cholesterol homeostasis and spermiation
| Autor: | Alberto Ruiz, Christine Dennefeld, Norbert B. Ghyselinck, Manuel Mark, Muriel Klopfenstein, Dean Bok, Nadège Vernet |
|---|---|
| Přispěvatelé: | Peney, Maité, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Neurobiology, University of California [Los Angeles] (UCLA), University of California (UC)-University of California (UC)-Jules Stein Eye Institute, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, University of California-University of California-Jules Stein Eye Institute |
| Rok vydání: | 2008 |
| Předmět: |
Male
Embryology Receptors Retinoic Acid Retinoic acid Gene Expression Retinoic acid receptor beta MESH: Mice Knockout chemistry.chemical_compound Mice MESH: Cholesterol 0302 clinical medicine Endocrinology Homeostasis MESH: Animals Retinoid X Receptor beta Mice Knockout 0303 health sciences [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] Retinoic Acid Receptor alpha Obstetrics and Gynecology Scavenger Receptors Class B Sertoli cell MESH: Gene Expression Regulation [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] Spermatids Cell biology medicine.anatomical_structure Cholesterol MESH: Homeostasis MESH: ATP-Binding Cassette Transporters Retinoid X receptor beta Dimerization ATP Binding Cassette Transporter 1 medicine.medical_specialty MESH: Spermatids MESH: Gene Expression Retinoid X receptor 03 medical and health sciences MESH: Sertoli Cells MESH: Mice Inbred C57BL Internal medicine medicine Animals MESH: Retinoid X Receptor beta MESH: Mice 030304 developmental biology MESH: Receptors Retinoic Acid Sertoli Cells Retinoid X receptor alpha Cell Biology Retinoid X receptor gamma MESH: Scavenger Receptors Class B MESH: Male Mice Inbred C57BL MESH: Dimerization Reproductive Medicine chemistry Gene Expression Regulation Retinoic acid receptor alpha ATP-Binding Cassette Transporters 030217 neurology & neurosurgery |
| Zdroj: | Journal of Reproduction and Fertility Journal of Reproduction and Fertility, 2008, 136 (5), pp.619-26. ⟨10.1530/REP-08-0235⟩ Journal of Reproduction and Fertility, Portland Press, 2008, 136 (5), pp.619-26. ⟨10.1530/REP-08-0235⟩ |
| ISSN: | 1741-7899 |
| DOI: | 10.1530/REP-08-0235⟩ |
| Popis: | Somatic, targeted inactivation of the retinoid X receptor beta gene (Rxrb) in Sertoli cells (SC; yieldingRxrbSer−/−mutants) leads to failure of spermatid release, accumulation of cholesterol esters and, subsequently, testis degeneration. These abnormalities are identical, in their nature and kinetics, to those observed upon inactivatingRxrbin the whole organism, thereby demonstrating that all reproductive functions of RXRB are carried out in SC. TheRxrbSer−/−testis degeneration is a consequence of a cholesterol ester cell overload occurring in SC in response to reduced ABCA1- and SCARB1-mediated cholesterol efflux. The failure of spermiation was also reported in mice lacking the retinoic acid (RA) receptor-α (RARA) in SC (RaraSer−/−mutants) and represents, in addition, a feature of vitamin A deficiency that can be readily induced in mice lacking the lecithin:retinol acyltransferase (Lrat−/−mutants). Altogether, these findings support the conclusion that RXRB heterodimerized with a RA-liganded RARA transduces signals required in SC for spermatid release. |
| Databáze: | OpenAIRE |
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